Biological parts for Kluyveromyces marxianus synthetic biology

Show simple item record

dc.contributor.author Rajkumar, Arun S.
dc.contributor.author Varela, Javier A.
dc.contributor.author Juergens, Hannes
dc.contributor.author Daran, Jean-Marc G.
dc.contributor.author Morrissey, John P.
dc.date.accessioned 2019-10-30T06:40:02Z
dc.date.available 2019-10-30T06:40:02Z
dc.date.issued 2019-05-07
dc.identifier.citation Rajkumar, A.S., Varela, J.A., Juergens, H., Daran, J.M. and Morrissey, J.P., 2019. Biological parts for Kluyveromyces marxianus synthetic biology. Frontiers in bioengineering and biotechnology, 7, 97. (15 pp.). DOI:10.3389/fbioe.2019.97 en
dc.identifier.volume 7 en
dc.identifier.startpage 1 en
dc.identifier.endpage 15 en
dc.identifier.uri http://hdl.handle.net/10468/8924
dc.identifier.doi 10.3389/fbioe.2019.97 en
dc.description.abstract Kluyveromyces marxianus is a non-conventional yeast whose physiology and metabolism lends itself to diverse biotechnological applications. While the wild-type yeast is already in use for producing fragrances and fermented products, the lack of standardised tools for its genetic and metabolic engineering prevent it from being used as a next-generation cell factory for bio-based chemicals. In this paper, we bring together and characterise a set of native K. marxianus parts for the expression of multiple genes for metabolic engineering and synthetic biology. All parts are cloned and stored according to the MoClo/Yeast Tool Kit standard for quick sharing and rapid construction. Using available genomic and transcriptomic data, we have selected promoters and terminators to fine-tune constitutive and inducible gene expression. The collection includes a number of known centromeres and autonomously replication sequences (ARS). We also provide a number of chromosomal integration sites selected for efficiency or visible phenotypes for rapid screening. Finally, we provide a single-plasmid CRISPR/Cas9 platform for genome engineering and facilitated gene targeting, and rationally create auxotrophic strains to expand the common range of selection markers available to K. marxianus. The curated and characterised tools we have provided in this kit will serve as a base to efficiently build next-generation cell factories from this alternative yeast. Plasmids containing all parts are available at Addgene for public distribution. en
dc.description.sponsorship Ministry of Economic Affairs, Agriculture and Innovation (FES subsidy to BE-Basic Research and Development) en
dc.format.mimetype application/pdf en
dc.language.iso en en
dc.publisher Frontiers Media S.A. en
dc.relation.uri https://www.frontiersin.org/articles/10.3389/fbioe.2019.00097
dc.rights © 2019 Rajkumar, Varela, Juergens, Daran and Morrissey. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. en
dc.rights.uri https://creativecommons.org/licenses/by/4.0/ en
dc.subject Kluyveromyces en
dc.subject Synthetic biology en
dc.subject Metabolic engineering en
dc.subject Genome engineering en
dc.subject Yeast en
dc.title Biological parts for Kluyveromyces marxianus synthetic biology en
dc.type Article (peer-reviewed) en
dc.internal.authorcontactother John Morrissey, School of Microbiology, University College Cork, Cork, Ireland. +353-21-490-3000 Email: j.morrissey@ucc.ie en
dc.internal.availability Full text available en
dc.description.version Published Version en
dc.contributor.funder Horizon 2020 en
dc.contributor.funder Seventh Framework Programme en
dc.contributor.funder BE-Basic Foundation en
dc.contributor.funder Ministry of Economic Affairs, Agriculture and Innovation, Netherlands en
dc.description.status Peer reviewed en
dc.identifier.journaltitle Frontiers in Bioengineering and Biotechnology en
dc.internal.IRISemailaddress j.morrissey@ucc.ie en
dc.identifier.articleid 97 en
dc.relation.project info:eu-repo/grantAgreement/EC/H2020::RIA/720824/EU/Model-Based Construction And Optimisation Of Versatile Chassis Yeast Strains For Production Of Valuable Lipid And Aromatic Compounds/CHASSY en
dc.relation.project info:eu-repo/grantAgreement/EC/FP7::SP3::PEOPLE/606795/EU/Yeast Cell Factories: Training Researchers to Apply Modern Post-Genomic Methods In Yeast Biotechnology/YEASTCELL en
dc.identifier.eissn 2296-4185


Files in this item

This item appears in the following Collection(s)

Show simple item record

© 2019 Rajkumar, Varela, Juergens, Daran and Morrissey. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. Except where otherwise noted, this item's license is described as © 2019 Rajkumar, Varela, Juergens, Daran and Morrissey. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
This website uses cookies. By using this website, you consent to the use of cookies in accordance with the UCC Privacy and Cookies Statement. For more information about cookies and how you can disable them, visit our Privacy and Cookies statement