Application of non-saccharomyces yeasts isolated from kombucha in the production of alcohol-free beer
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Date
2018-08-17
Authors
Bellut, Konstantin
Michel, Maximilian
Zarnkow, Martin
Hutzler, Mathias
Jacob, Fritz
De Schutter, David P.
Daenen, Luk
Lynch, Kieran M.
Zannini, Emanuele
Arendt, Elke K.
Journal Title
Journal ISSN
Volume Title
Publisher
MDPI AG
Published Version
Abstract
Alcohol-free beer (AFB) is no longer just a niche product in the beer market. For brewers, this product category offers economic benefits in the form of a growing market and often a lower tax burden and enables brewers to extend their product portfolio and promote responsible drinking. Non-Saccharomyces yeasts are known for their flavor-enhancing properties in food fermentations, and their prevailing inability to ferment maltose and maltotriose sets a natural fermentation limit and can introduce a promising approach in the production of AFB (≤0.5% v/v). Five strains isolated from kombucha, Hanseniaspora valbyensis, Hanseniaspora vineae, Torulaspora delbrueckii, Zygosaccharomyces bailii and Zygosaccharomyces kombuchaensis were compared to a commercially applied AFB strain Saccharomycodes ludwigii and a Saccharomyces cerevisiae brewer’s yeast. The strains were characterized for their sugar utilization, phenolic off-flavors, hop sensitivity and flocculation. Trial fermentations were analyzed for extract reduction, ethanol formation, pH drop and final beers were analyzed for amino acids utilization and fermentation by-products. The performance of non-Saccharomyces strains and the commercial AFB strain were comparable during fermentation and production of fermentation by-products. An experienced sensory panel could not discriminate between the non-Saccharomyces AFB and the one produced with the commercial AFB strain, therefore indicating their suitability in AFB brewing.
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Keywords
Non-alcoholic beer , Non-conventional yeast , Non-Saccharomyces , Brewing , Fermentation , Yeast characterization , Sensory
Citation
Bellut, K., Michel, M., Zarnkow, M., Hutzler, M., Jacob, F., De Schutter, D., Daenen, L., Lynch, K., Zannini, E. and Arendt, E., 2018. Application of Non-Saccharomyces Yeasts Isolated from Kombucha in the Production of Alcohol-Free Beer. Fermentation, 4(3), (66). DOI:10.3390/fermentation4030066