Identification and characterisation of capidermicin, a novel bacteriocin produced by Staphylococcus capitis

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dc.contributor.author Lynch, David
dc.contributor.author O'Connor, Paula M.
dc.contributor.author Cotter, Paul D.
dc.contributor.author Hill, Colin
dc.contributor.author Field, Des
dc.contributor.author Begley, Máire
dc.date.accessioned 2019-12-03T11:42:44Z
dc.date.available 2019-12-03T11:42:44Z
dc.date.issued 2019-10-16
dc.identifier.citation Lynch, D., O’Connor, P. M., Cotter, P. D., Hill, C., Field, D. and Begley, M. (2019) 'Identification and characterisation of capidermicin, a novel bacteriocin produced by Staphylococcus capitis', PLOS ONE, 14(10), e0223541. (17pp.) doi: 10.1371/journal.pone.0223541 en
dc.identifier.volume 14 en
dc.identifier.issued 10 en
dc.identifier.startpage 1 en
dc.identifier.endpage 17 en
dc.identifier.uri http://hdl.handle.net/10468/9297
dc.identifier.doi 10.1371/journal.pone.0223541 en
dc.description.abstract One hundred human-derived coagulase negative staphylococci (CoNS) were screened for antimicrobial activity using agar-based deferred antagonism assays with a range of indicator bacteria. Based on the findings of the screen and subsequent well assays with cell free supernatants and whole cell extracts, one strain, designated CIT060, was selected for further investigation. It was identified as Staphylococcus capitis and herein we describe the purification and characterisation of the novel bacteriocin that the strain produces. This bacteriocin which we have named capidermicin was extracted from the cell-free supernatant of S. capitis CIT060 and purified to homogeneity using reversed-phase high performance liquid chromatography (RP-HPLC). Matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometric (MS) analysis revealed that the capidermicin peptide has a mass of 5,464 Da. Minimal inhibitory concentration (MIC) experiments showed that capidermicin was active in the micro-molar range against all the Gram-positive bacteria that were tested. Antimicrobial activity was retained over a range of pHs (2–11) and temperatures (10–121°C x 15 mins). The draft genome sequence of S. capitis CIT060 was determined and the genes predicted to be involved in the biosynthesis of capidermicin were identified. These genes included the predicted capidermicin precursor gene, and genes that are predicted to encode a membrane transporter, an immunity protein and a transcriptional regulator. Homology searches suggest that capidermicin is a novel member of the family of class II leaderless bacteriocins. en
dc.format.mimetype application/pdf en
dc.language.iso en en
dc.publisher PLoS en
dc.rights ©2019 Lynch et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited en
dc.rights.uri http://creativecommons.org/licenses/by/4.0/ en
dc.subject Capidermicin en
dc.subject Bacteriocin en
dc.subject Staphylococcus capitis en
dc.subject Coagulase negative staphylococci (CoNS) en
dc.title Identification and characterisation of capidermicin, a novel bacteriocin produced by Staphylococcus capitis en
dc.type Article (peer-reviewed) en
dc.internal.authorcontactother Des Field, School of Microbiology and APC Microbiome Ireland, University College Cork, Cork, Ireland. +353-21-490-3000 Email:des.field@ucc.ie en
dc.internal.availability Full text available en
dc.description.version Published Version en
dc.contributor.funder Cork Institute of Technology en
dc.description.status Peer reviewed en
dc.identifier.journaltitle PLOS One en
dc.internal.IRISemailaddress des.field@ucc.ie en
dc.identifier.articleid e0223541 en
dc.identifier.eissn 1932-6203


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©2019 Lynch et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited Except where otherwise noted, this item's license is described as ©2019 Lynch et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
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