Orally administered CLA ameliorates DSS-induced colitis in mice via intestinal barrier improvement, oxidative stress reduction, inflammatory cytokine and gut microbiota modulation
Chen, Yang; Yang, Bo; Ross, R. Paul; Jin, Yan; Stanton, Catherine; Zhao, Jianxin; Zhang, Hao; Chen, Wei
Date:
2019-11-06
Copyright:
© 2019, American Chemical Society. This document is the Accepted Manuscript version of a Published Work that appeared in final form in Journal of Agricultural and Food Chemistry, © American Chemical Society, after peer review and technical editing by the publisher. To access the final edited and published work see https://pubs.acs.org/doi/abs/10.1021/acs.jafc.9b05744
Full text restriction information:
Access to this article is restricted until 12 months after publication by request of the publisher.
Restriction lift date:
2020-11-06
Citation:
Chen, Y., Yang, B., Ross, R. P., Jin, Y., Stanton, C., Zhao, J., Zhang, H. and Chen, W. (2019) 'Orally administered CLA ameliorates DSS-induced colitis in mice via intestinal barrier improvement, oxidative stress reduction, inflammatory cytokine and gut microbiota modulation', Journal of Agricultural and Food Chemistry, 67(48), pp. 13282-13298. doi: 10.1021/acs.jafc.9b05744
Abstract:
Dietary supplementation with CLA has been reported to alleviate the effect of colitis in mice, but the mechanisms involved need further exploration. The study aimed to investigate how orally administered CLA alleviates DSS-induced colitis in mice. CLA was administered at five different doses: 40 mg/d, 20 mg/d, 10 mg/d, 5 mg/d and 2.5 mg/d. Doses of CLA at 10 mg/d and higher alleviated colitis symptoms and reduced inflammation induced by DSS, in which 40 mg/d, 20 mg/d and 10 mg/d CLA significantly increased the concentration of MUC2 and goblet cells, but neither 5 mg/d CLA nor 2.5 mg/d CLA had any effects. Meanwhile, 40 mg/d CLA and 20 mg/d CLA treatments significantly up-regulated the concentration of tight junction proteins (ZO-1, occludin and claudin-3) and ameliorated epithelial apoptosis caused by DSS. Moreover, oxidative stress-related enzymes (SOD, GSH-PX, CAT) and inflammatory cytokines (TNF-a, IL-10, IL-6) were modulated by 40 mg/d CLA and 20 mg/d CLA. Furthermore, 40 mg/d CLA rebalanced the gut microbiota damaged by DSS, including reducing Bacteroides and increasing Bifidobacterium and Odoribacter. In conclusion, CLA supplementation alleviated DSS-induced colitis in a dose-dependent manner by modulating inflammatory cytokines and oxidation stress, maintaining the mucosal barrier and reverting microbiota changes.
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