dc.contributor.author |
Martín, Guiomar |
|
dc.contributor.author |
Veciana, Nil |
|
dc.contributor.author |
Boix, Marc |
|
dc.contributor.author |
Rovira, Arnau |
|
dc.contributor.author |
Henriques, Rossana |
|
dc.contributor.author |
Monte, Elena |
|
dc.date.accessioned |
2020-05-13T12:10:18Z |
|
dc.date.available |
2020-05-13T12:10:18Z |
|
dc.date.issued |
2020-05-07 |
|
dc.identifier.citation |
Martín, G., Veciana, N., Boix, M., Rovira, A., Henriques, R. and Monte, E. (2020) 'The photoperiodic response of hypocotyl elongation involves regulation of CDF1 and CDF5 activity', Physiologia Plantarum. doi: 10.1111/ppl.13119 |
en |
dc.identifier.issn |
0031-9317 |
|
dc.identifier.uri |
http://hdl.handle.net/10468/9947 |
|
dc.identifier.doi |
10.1111/ppl.13119 |
en |
dc.description.abstract |
Hypocotyl elongation relies on directional cell expansion, a process under light and circadian clock control. Under short photoperiods (SD), hypocotyl elongation in Arabidopsis thaliana follows a rhythmic pattern, a process in which circadian morning-to-midnight waves of the transcriptional repressors PSEUDO-RESPONSE REGULATORS (PRRs) jointly gate PHYTOCHROME-INTERACTING FACTOR (PIF) activity to dawn. Previously, we described CYCLING DOF FACTOR 5 (CDF5) as a target of this antagonistic PRR/PIF dynamic interplay. Under SD, PIFs induce CDF5 accumulation specifically at dawn, when it promotes the expression of positive cell elongation regulators such as YUCCA8 to induce growth. In contrast to SD, hypocotyl elongation under long days (LD) is largely reduced. Here, we examine whether CDF5 is an actor in this photoperiod specific regulation. We report that transcription of CDF5 is robustly induced in SD compared to LD, in accordance with PIFs accumulating to higher levels in SD, and in contrast to other members of the CDF family, whose expression is mainly clock regulated and have similar waveforms in SD and LD. Notably, when CDF5 was constitutively expressed under LD, CDF5 protein accumulated to levels comparable to SD but was inactive in promoting cell elongation. Similar results were observed for CDF1. Our findings indicate that both CDFs can promote cell elongation specifically in shorter photoperiods, however their activity in LD is inhibited at the post-translational level. These data not only expand our understanding of the biological role of CDF transcription factors, but also identify a previously unrecognized regulatory layer in the photoperiodic response of hypocotyl elongation. |
en |
dc.description.sponsorship |
Ministerio de Economía, Industria y Competitividad, Gobierno de España (BIO2015-72161-EXP; BIO2015-70812-ERC; RYC-2011-09220; Centro de Excelencia Severo Ochoa 2016–2019” award SEV-2015-0533); European Commission (PCIG2012-GA-2012-334052) University College Cork (School of Biological, Earth and Environmental Sciences start-up funding); European Regional Development Fund / Ministerio de Ciencia, Innovación y Universidades (Project References BIO2015-68460-P and PGC2018-099987-B-I00); Generalitat de Catalunya (CERCA Programme: Project Reference 2017SGR-718) |
en |
dc.format.mimetype |
application/pdf |
en |
dc.language.iso |
en |
en |
dc.publisher |
John Wiley & Sons, Inc. |
en |
dc.rights |
© 2020, John Wiley & Sons, Inc. This is the peer reviewed version of the following article: Martín, G., Veciana, N., Boix, M., Rovira, A., Henriques, R. and Monte, E. (2020) 'The photoperiodic response of hypocotyl elongation involves regulation of CDF1 and CDF5 activity', Physiologia Plantarum, doi: 10.1111/ppl.13119, which has been published in final form at https://doi.org/10.1111/ppl.13119. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions. |
en |
dc.subject |
CDF transcription factors |
en |
dc.subject |
Hypocotyl elongation |
en |
dc.subject |
Photoperiodic response |
en |
dc.title |
The photoperiodic response of hypocotyl elongation involves regulation of CDF1 and CDF5 activity |
en |
dc.type |
Article (peer-reviewed) |
en |
dc.internal.authorcontactother |
Rossana Henriques, Biological, Earth and Environmental Sciences, University College Cork, Cork, Ireland. +353-21-490-3000 Email: rossana.henriques@ucc.ie |
en |
dc.internal.availability |
Full text available |
en |
dc.check.info |
Access to this article is restricted until 12 months after publication by request of the publisher. |
en |
dc.check.date |
2021-05-07 |
|
dc.date.updated |
2020-05-13T09:51:03Z |
|
dc.description.version |
Accepted Version |
en |
dc.internal.rssid |
514147484 |
|
dc.internal.pmid |
32379360 |
|
dc.contributor.funder |
Ministerio de Economía, Industria y Competitividad, Gobierno de España
|
en |
dc.contributor.funder |
European Commission
|
en |
dc.contributor.funder |
University College Cork
|
en |
dc.contributor.funder |
Ministerio de Ciencia, Innovación y Universidades
|
en |
dc.contributor.funder |
European Regional Development Fund
|
en |
dc.contributor.funder |
Generalitat de Catalunya
|
en |
dc.description.status |
Peer reviewed |
en |
dc.identifier.journaltitle |
Physiologia Plantarum |
en |
dc.internal.copyrightchecked |
Yes |
|
dc.internal.licenseacceptance |
Yes |
en |
dc.internal.IRISemailaddress |
rossana.henriques@ucc.ie |
en |
dc.internal.bibliocheck |
In press. Check volume / issue / page range. Amend citation and copyright statement accordingly. |
en |
dc.identifier.eissn |
1399-3054 |
|