The photoperiodic response of hypocotyl elongation involves regulation of CDF1 and CDF5 activity

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dc.contributor.author Martí­n, Guiomar
dc.contributor.author Veciana, Nil
dc.contributor.author Boix, Marc
dc.contributor.author Rovira, Arnau
dc.contributor.author Henriques, Rossana
dc.contributor.author Monte, Elena
dc.date.accessioned 2020-05-13T12:10:18Z
dc.date.available 2020-05-13T12:10:18Z
dc.date.issued 2020-05-07
dc.identifier.citation Martín, G., Veciana, N., Boix, M., Rovira, A., Henriques, R. and Monte, E. (2020) 'The photoperiodic response of hypocotyl elongation involves regulation of CDF1 and CDF5 activity', Physiologia Plantarum. doi: 10.1111/ppl.13119 en
dc.identifier.issn 0031-9317
dc.identifier.uri http://hdl.handle.net/10468/9947
dc.identifier.doi 10.1111/ppl.13119 en
dc.description.abstract Hypocotyl elongation relies on directional cell expansion, a process under light and circadian clock control. Under short photoperiods (SD), hypocotyl elongation in Arabidopsis thaliana follows a rhythmic pattern, a process in which circadian morning-to-midnight waves of the transcriptional repressors PSEUDO-RESPONSE REGULATORS (PRRs) jointly gate PHYTOCHROME-INTERACTING FACTOR (PIF) activity to dawn. Previously, we described CYCLING DOF FACTOR 5 (CDF5) as a target of this antagonistic PRR/PIF dynamic interplay. Under SD, PIFs induce CDF5 accumulation specifically at dawn, when it promotes the expression of positive cell elongation regulators such as YUCCA8 to induce growth. In contrast to SD, hypocotyl elongation under long days (LD) is largely reduced. Here, we examine whether CDF5 is an actor in this photoperiod specific regulation. We report that transcription of CDF5 is robustly induced in SD compared to LD, in accordance with PIFs accumulating to higher levels in SD, and in contrast to other members of the CDF family, whose expression is mainly clock regulated and have similar waveforms in SD and LD. Notably, when CDF5 was constitutively expressed under LD, CDF5 protein accumulated to levels comparable to SD but was inactive in promoting cell elongation. Similar results were observed for CDF1. Our findings indicate that both CDFs can promote cell elongation specifically in shorter photoperiods, however their activity in LD is inhibited at the post-translational level. These data not only expand our understanding of the biological role of CDF transcription factors, but also identify a previously unrecognized regulatory layer in the photoperiodic response of hypocotyl elongation. en
dc.description.sponsorship Ministerio de Economía, Industria y Competitividad, Gobierno de España (BIO2015-72161-EXP; BIO2015-70812-ERC; RYC-2011-09220; Centro de Excelencia Severo Ochoa 2016–2019” award SEV-2015-0533); European Commission (PCIG2012-GA-2012-334052) University College Cork (School of Biological, Earth and Environmental Sciences start-up funding); European Regional Development Fund / Ministerio de Ciencia, Innovación y Universidades (Project References BIO2015-68460-P and PGC2018-099987-B-I00); Generalitat de Catalunya (CERCA Programme: Project Reference 2017SGR-718) en
dc.format.mimetype application/pdf en
dc.language.iso en en
dc.publisher John Wiley & Sons, Inc. en
dc.rights © 2020, John Wiley & Sons, Inc. This is the peer reviewed version of the following article: Martín, G., Veciana, N., Boix, M., Rovira, A., Henriques, R. and Monte, E. (2020) 'The photoperiodic response of hypocotyl elongation involves regulation of CDF1 and CDF5 activity', Physiologia Plantarum, doi: 10.1111/ppl.13119, which has been published in final form at https://doi.org/10.1111/ppl.13119. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions. en
dc.subject CDF transcription factors en
dc.subject Hypocotyl elongation en
dc.subject Photoperiodic response en
dc.title The photoperiodic response of hypocotyl elongation involves regulation of CDF1 and CDF5 activity en
dc.type Article (peer-reviewed) en
dc.internal.authorcontactother Rossana Henriques, Biological, Earth and Environmental Sciences, University College Cork, Cork, Ireland. +353-21-490-3000 Email: rossana.henriques@ucc.ie en
dc.internal.availability Full text available en
dc.check.info Access to this article is restricted until 12 months after publication by request of the publisher. en
dc.check.date 2021-05-07
dc.date.updated 2020-05-13T09:51:03Z
dc.description.version Accepted Version en
dc.internal.rssid 514147484
dc.internal.pmid 32379360
dc.contributor.funder Ministerio de Economía, Industria y Competitividad, Gobierno de España en
dc.contributor.funder European Commission en
dc.contributor.funder University College Cork en
dc.contributor.funder Ministerio de Ciencia, Innovación y Universidades en
dc.contributor.funder European Regional Development Fund en
dc.contributor.funder Generalitat de Catalunya en
dc.description.status Peer reviewed en
dc.identifier.journaltitle Physiologia Plantarum en
dc.internal.copyrightchecked Yes
dc.internal.licenseacceptance Yes en
dc.internal.IRISemailaddress rossana.henriques@ucc.ie en
dc.internal.bibliocheck In press. Check volume / issue / page range. Amend citation and copyright statement accordingly. en
dc.identifier.eissn 1399-3054


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