Raffinocyclicin is a novel plasmid-encoded circular bacteriocin produced by Lactococcus raffinolactis with broad-spectrum activity against many gram-positive food pathogens

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dc.contributor.authorde Farias, Felipe Micelien
dc.contributor.authorO'Connor, Paula M.en
dc.contributor.authorButtimer, Colinen
dc.contributor.authorKamilari, Elenien
dc.contributor.authorSoria, Maria Ceciliaen
dc.contributor.authorJohnson, Crystalen
dc.contributor.authorDeliephan, Aiswariyaen
dc.contributor.authorHill, Daraghen
dc.contributor.authorFursenko, Oxanaen
dc.contributor.authorWiese, Jonathanen
dc.contributor.authorDraper, Lorraine A.en
dc.contributor.authorStanton, Catherineen
dc.contributor.authorHill, Colinen
dc.contributor.authorRoss, R. Paulen
dc.contributor.funderScience Foundation Irelanden
dc.contributor.funderH. J. Heinz Companyen
dc.contributor.funderEuropean Commissionen
dc.date.accessioned2025-09-03T15:01:46Z
dc.date.available2025-09-03T15:01:46Z
dc.date.issued2024-09-18en
dc.description.abstractThis study describes the discovery and characterization of raffinocyclicin, a novel plasmid-encoded circular bacteriocin, produced by the raw milk isolate Lactococcus raffinolactis APC 3967. This bacteriocin has a molecular mass of 6,092 Da and contains 61 amino acids with a three-amino acid leader peptide. It shows the highest identity to the circular bacteriocins bacicyclicin XIN-1 (42.62%), aureocyclicin 4185 (42.62%), and garvicin ML (41.53%). A broad inhibitory spectrum includes strains from Staphylococcus, Enterococcus, Streptococcus, Micrococcus, Lactobacillus, Leuconostoc, and Listeria, in addition to a pronounced inhibitory effect against Lactococcus and Clostridium. It displays low sensitivity to trypsin, most likely as a result of its circular nature. The raffinocyclicin gene cluster is composed of 10 genes: 6 core genes, genes encoding an accessory three-component ABC transporter (rafCDE), and a putative transcriptional regulator related to the MutR family. A lack of inhibitory activity in the cell-free supernatant combined with the pronounced activity of cell extracts suggests that the majority of raffinocyclicin is associated with the cell rather than being released to the extracellular environment. This is the first report of a bacteriocin produced by the L. raffinolactis species.en
dc.description.statusPeer revieweden
dc.description.versionPublished Versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.articleide00809-24en
dc.identifier.citationMiceli De Farias, F., O'Connor, P. M., Buttimer, C., Kamilari, E., Soria, M. C., Johnson, C. N. , Deliephan, A., Hill, D., Fursenko, O., Wiese, J., Draper, L.A., Stanton, C., Hill, C., and Ross, R. P. (2024) 'Raffinocyclicin is a novel plasmid-encoded circular bacteriocin produced by Lactococcus raffinolactis with broad-spectrum activity against many gram-positive food pathogens', Applied and Environmental Microbiology, 90 (9) e00809-24 (19pp). https://doi: 10.1128/aem.00809-24en
dc.identifier.doi10.1128/aem.00809-24en
dc.identifier.eissn1098-5336en
dc.identifier.endpage19en
dc.identifier.issn0099-2240en
dc.identifier.issued9en
dc.identifier.journaltitleApplied and Environmental Microbiologyen
dc.identifier.startpage1en
dc.identifier.urihttps://hdl.handle.net/10468/17822
dc.identifier.volume90en
dc.language.isoenen
dc.publisherAmerican Society for Microbiologyen
dc.relation.projectinfo:eu-repo/grantAgreement/SFI/Research Centres Programme/12/RC/2273/IE/Alimentary Pharmabiotic Centre (APC) - Interfacing Food & Medicine/en
dc.relation.projectinfo:eu-repo/grantAgreement/EC/HE::ERC::HORIZON-ERC/101054719/EU/Bacteriocins from interbacterial warfare as antibiotic alternative/BACtheWINNERen
dc.rights© 2024 Miceli de Farias, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.en
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.subjectCircular bacteriocinen
dc.subjectAntimicrobial peptideen
dc.subjectRaffinocyclicinen
dc.subjectAntimicrobial activityen
dc.subjectLactococcus raffinolactisen
dc.titleRaffinocyclicin is a novel plasmid-encoded circular bacteriocin produced by Lactococcus raffinolactis with broad-spectrum activity against many gram-positive food pathogensen
dc.typeArticle (peer-reviewed)en
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