Raffinocyclicin is a novel plasmid-encoded circular bacteriocin produced by Lactococcus raffinolactis with broad-spectrum activity against many gram-positive food pathogens
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Date
2024-09-18
Authors
de Farias, Felipe Miceli
O’Connor, Paula M.
Buttimer, Colin
Kamilari, Eleni
Soria, Maria Cecilia
Johnson, Crystal
Deliephan, Aiswariya
Hill, Daragh
Fursenko, Oxana
Wiese, Jonathan
Journal Title
Journal ISSN
Volume Title
Publisher
American Society for Microbiology
Published Version
Abstract
This study describes the discovery and characterization of raffinocyclicin, a novel plasmid-encoded circular bacteriocin, produced by the raw milk isolate Lactococcus raffinolactis APC 3967. This bacteriocin has a molecular mass of 6,092 Da and contains 61 amino acids with a three-amino acid leader peptide. It shows the highest identity to the circular bacteriocins bacicyclicin XIN-1 (42.62%), aureocyclicin 4185 (42.62%), and garvicin ML (41.53%). A broad inhibitory spectrum includes strains from Staphylococcus, Enterococcus, Streptococcus, Micrococcus, Lactobacillus, Leuconostoc, and Listeria, in addition to a pronounced inhibitory effect against Lactococcus and Clostridium. It displays low sensitivity to trypsin, most likely as a result of its circular nature. The raffinocyclicin gene cluster is composed of 10 genes: 6 core genes, genes encoding an accessory three-component ABC transporter (rafCDE), and a putative transcriptional regulator related to the MutR family. A lack of inhibitory activity in the cell-free supernatant combined with the pronounced activity of cell extracts suggests that the majority of raffinocyclicin is associated with the cell rather than being released to the extracellular environment. This is the first report of a bacteriocin produced by the L. raffinolactis species.
Description
Keywords
Circular bacteriocin , Antimicrobial peptide , Raffinocyclicin , Antimicrobial activity , Lactococcus raffinolactis
Citation
Miceli De Farias, F., O’Connor, P. M., Buttimer, C., Kamilari, E., Soria, M. C., Johnson, C. N. , Deliephan, A., Hill, D., Fursenko, O., Wiese, J., Draper, L.A., Stanton, C., Hill, C., and Ross, R. P. (2024) 'Raffinocyclicin is a novel plasmid-encoded circular bacteriocin produced by Lactococcus raffinolactis with broad-spectrum activity against many gram-positive food pathogens', Applied and Environmental Microbiology, 90 (9) e00809-24 (19pp). https://doi: 10.1128/aem.00809-24