Microbiota and metabolome dynamics induced by Shiga toxin-producing E. coli in an in vitro model of an infant’s colon

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dc.contributor.authorIzquierdo, Marianaen
dc.contributor.authorO’Sullivan, Deborahen
dc.contributor.authorUriot, Ophélieen
dc.contributor.authorBrun, Morganeen
dc.contributor.authorDurif, Claudeen
dc.contributor.authorDenis, Sylvainen
dc.contributor.authorGallardo, Pabloen
dc.contributor.authorGahan, Cormac G. M.en
dc.contributor.authorEtienne-Mesmin, Lucieen
dc.contributor.authorBlanquet-Diot, Stéphanieen
dc.contributor.authorFarfan, Mauricio J.en
dc.contributor.funderAgencia Nacional de Investigación y Desarrolloen
dc.contributor.funderEvaluation-orientation de la Coopération Scientifiqueen
dc.contributor.funderECOSen
dc.contributor.funderFondo Nacional de Desarrollo Científico y Tecnológicoen
dc.contributor.funderHorizon 2020en
dc.contributor.funderVicerrectoría de Investigación y Desarrolloen
dc.contributor.funderUniversidad de Chileen
dc.date.accessioned2025-05-28T09:00:53Z
dc.date.available2025-05-28T09:00:53Z
dc.date.issued2025-04-14en
dc.description.abstractShiga toxin-producing Escherichia coli (STEC) is a major food-borne pathogen causing human diseases ranging from diarrhea to life-threatening complications, mainly in young children. Colonization, virulence, and interactions of STEC strains with human gut microbiota are pivotal during infection but remain poorly described, particularly in children, the most affected population. In this work, we evaluated changes in the microbiota and metabolome composition in the in vitro gut model: Toddler ARtificial COLon (T-ARCOL) infected with EHEC O157:H7 strain EDL 933. Stool samples collected from children with STEC-positive diarrhea and stool from the same children after recovery from the diarrheal episode (n=5) were used to inoculate the T-ARCOL model. STEC colonization was progressively reduced throughout fermentation in T-ARCOL with diarrhea or recovery fecal samples. Beta diversity showed that the diarrhea-associated microbiota was significantly distinct from the recovery microbiota and exhibited a lower α-diversity. In contrast to recovery conditions, diarrheal conditions were characterized by an increased abundance of potential pathobionts such as members of the Clostridiaceae family and higher acetate, succinate, and N-acetylneuraminic acid levels. Our results provide new evidence of the impact of EHEC in the microbiota and metabolome dynamics in an in vitro gut model that could be useful in understanding their physiopathology in this at-risk population, considering inter-individual variabilities in gut microbiota.en
dc.description.sponsorshipECOS Sud (Action C19B02); Fondo Nacional de Desarrollo Científico y Tecnológico/Agencia Nacional de Investigación y Desarrollo (Grants 1200994; 1240551); Vicerrectoría de Investigación y Desarrollo (VID) - Universidad de Chile (AYV 053/01-22); Agencia Nacional de Investigación y Desarrollo (Doctoral fellowship 21201883)en
dc.description.statusPeer revieweden
dc.description.versionPublished Versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.citationIzquierdo, M., O'Sullivan, D., Uriot, O., Brun, M., Durif, C., Denis, S., Gallardo, P., Gahan, C. G., Etienne-Mesmin, L., Blanquet-Diot, S. and Farfan, M. J. (2025) 'Microbiota and metabolome dynamics induced by Shiga toxin-producing E. coli in an in vitro model of an infant's colon', Microbial Cell, 12(1), pp.76-92. https://doi.org/10.15698/mic2025.04.847en
dc.identifier.doi10.15698/mic2025.04.847en
dc.identifier.eissn2311-2638en
dc.identifier.endpage92en
dc.identifier.issued1en
dc.identifier.journaltitleMicrobial Cellen
dc.identifier.startpage76en
dc.identifier.urihttps://hdl.handle.net/10468/17584
dc.identifier.volume12en
dc.language.isoenen
dc.publisherShared Science Publishers OGen
dc.relation.ispartofMicrobial Cellen
dc.relation.projectinfo:eu-repo/grantAgreement/EC/H2020::MSCA-ITN-ETN/956279/EU/Colonization resistance: training and research towards microbiome derived solutions to foodborne disease/COL_RESen
dc.rights© 2025, the Authors. Licensed under a Creative Commons Attribution 4.0 International License.en
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/en
dc.subjectShiga toxin-producing Escherichia coli (STEC)en
dc.subjectDiarrheaen
dc.subjectGut microbiotaen
dc.subjectGut metabolomeen
dc.subjectToddler artificial colon model (T-ARCOL)en
dc.titleMicrobiota and metabolome dynamics induced by Shiga toxin-producing E. coli in an in vitro model of an infant’s colonen
dc.typeArticle (peer-reviewed)en
dc.typejournal-articleen
oaire.citation.volume12en
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