A plasmid-encoded putative glycosyltransferase is involved in hop tolerance and beer spoilage in Lactobacillus brevis

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dc.contributor.authorFeyereisen, Marine
dc.contributor.authorMahony, Jennifer
dc.contributor.authorO'Sullivan, Tadhg
dc.contributor.authorBoer, Viktor
dc.contributor.authorvan Sinderen, Douwe
dc.contributor.funderIrish Research Councilen
dc.contributor.funderScience Foundation Irelanden
dc.date.accessioned2020-05-14T16:11:51Z
dc.date.available2020-05-14T16:11:51Z
dc.date.issued2020-01-21
dc.date.updated2020-05-14T15:59:32Z
dc.description.abstractLactobacillus brevis beer-spoiling strains harbor plasmids that contain genes such as horA, horC, and hitA which are known to confer hop tolerance. The L. brevis beer-spoiling strain UCCLBBS124, which possesses four plasmids, was treated with novobiocin, resulting in the isolation of UCCLBBS124 derivatives exhibiting hop sensitivity and an inability to grow in beer. One selected derivative was shown to have lost a single plasmid, here designated UCCLBBS124_D, which harbors the UCCLBBS124_pD0015 gene, predicted to encode a glycosyltransferase. Hop tolerance and growth in beer were restored when UCCLBBS124_pD0015 was introduced in one of these hop-sensitive derivatives on a plasmid. We hypothesize that this gene modifies the surface composition of the polysaccharide cell wall, conferring protection against hop compounds. Furthermore, the introduction of this gene in trans in L. brevis UCCLB521, a strain that cannot grow in and spoil beer, was shown to furnish the resulting strain with the ability to grow in beer, while its expression also conferred phage resistance. This study underscores how the acquisition of certain mobile genetic elements plays a role in hop tolerance and beer spoilage for strains of this bacterial species.IMPORTANCELactobacillus brevis is a member of the lactic acid bacteria and is often reported as the causative agent of food or beverage spoilage, in particular, that of beer. Bacterial spoilage of beer may result in product withdrawal or recall, with concomitant economic losses for the brewing industry. A very limited number of genes involved in beer spoilage have been identified and primarily include those involved in hop resistance, such as horA, hitA, and horC However, since none of these genes are universal, it is clear that there are likely (many) other molecular players involved in beer spoilage. Here, we report on the importance of a plasmid-encoded glycosyltransferase associated with beer spoilage by L. brevis that is involved in hop tolerance. The study highlights the complexity of the genetic requirements to facilitate beer spoilage and the role of multiple key players in this process.en
dc.description.sponsorshipIrish Research Council (Enterprise Partnership Scheme postgraduate scholarship (reference no. EPSPG/2015/7); Science Foundation Ireland ((reference no. 450 13/IA/1953), Starting Investigator Research Grant (SIRG) (reference no. 15/SIRG/3430))en
dc.description.statusPeer revieweden
dc.description.versionAccepted Versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.articleide02268-19en
dc.identifier.citationFeyereisen, M., Mahony, J., O'Sullivan, T. and van Sinderen, D. (2020) 'A Plasmid-Encoded Putative Glycosyltransferase Is Involved in Hop Tolerance and Beer Spoilage in Lactobacillus brevis', Applied and Environmental Microbiology, 86(3), e02268-19, (10 pp). doi: 10.1128/AEM.02268-19en
dc.identifier.doi10.1128/AEM.02268-19en
dc.identifier.endpage10en
dc.identifier.issn1098-5336
dc.identifier.issued3en
dc.identifier.journaltitleApplied and Environmental Microbiologyen
dc.identifier.startpage1en
dc.identifier.urihttps://hdl.handle.net/10468/9955
dc.identifier.volume86en
dc.language.isoenen
dc.publisherAmerican Society for Microbiologyen
dc.relation.projectinfo:eu-repo/grantAgreement/SFI/SFI Starting Investigator Research Grant (SIRG)/15/SIRG/3430/IE/Phage-host interactome of the dairy bacterium Streptococcus thermophilus (PHIST)/en
dc.relation.urihttps://aem.asm.org/content/86/3/e02268-19
dc.rights© 2020 American Society for Microbiology. All Rights Reserved.en
dc.subjectLactic acid bacteriaen
dc.subjectPlasmid resistanceen
dc.subjectHorAen
dc.subjectCell wallen
dc.subjectPolysaccharideen
dc.subjectPhageen
dc.subjectBeer spoilageen
dc.subjectLactobacillus brevisen
dc.subjectMicrobiologyen
dc.titleA plasmid-encoded putative glycosyltransferase is involved in hop tolerance and beer spoilage in Lactobacillus brevisen
dc.typeArticle (peer-reviewed)en
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