Triangular gold nanoparticles modify shell characteristics and increase antioxidant enzyme activities in the clam Ruditapes decussatus
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Accepted version
Date
2018-04-07
Authors
Abdelhafidh, Khazri
Badreddine, Sellami
Mezni, Amine
Mouhamed, Dellali
Wiem, Saidani
Imen, Bouzidi
Sheehan, David
Mahmoudi, Ezzeddine
Hamouda, Beyrem
Journal Title
Journal ISSN
Volume Title
Publisher
Taylor & Francis
Published Version
Abstract
Context: Nanoparticles may cause adverse environmental effects but there is limited information on their interactions with marine organisms. Objective: Our aim was to examine the effects of triangular gold nanoparticles (Tr-Au NPs) on the clam, Ruditapes decussatus. Materials and methods: Clams were exposed to Tr-Au1 = 5 µg/L and Tr-Au2 = 10 µg/L for 2 and 7 days. Effects on shell structure were investigated. Superoxide dismutase (SOD), catalase (CAT), glutathione transferase (GST) activities, protein carbonyl levels and malondialdehyde content were used to assess biochemical status. Results: Transmission electron microscopy (TEM) and electron dispersive X-ray microanalysis (EDX) showed that Tr-Au NPs modified shell structure and morphology. Tr-Au NPs size increased forming aggregate particles. Tr-Au NPs increased SOD, CAT and GST activities in gill and digestive gland in a concentration- and time-dependent manner indicating defence against oxidative stress. Enhanced lipid peroxidation and protein carbonyl levels confirmed oxidative stress. Conclusion: Tr-Au NPs cause oxidative stress and affect shell structure of clams. These findings may have relevance to other marine species.
Description
Keywords
Oxidative stress , Biomarkers , Biomonitoring , Bivalve
Citation
Abdelhafidh, K., Badreddine, S., Mezni, A., Mouhamed, D., Wiem, S., Imen, B., David, S., Mahmoudi, E. and Hamouda, B. (2018) 'Triangular gold nanoparticles modify shell characteristics and increase antioxidant enzyme activities in the clam Ruditapes decussatus', Biomarkers, In Press, doi: 10.1080/1354750X.2018.1463565
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© 2018 Informa UK Limited, trading as Taylor & Francis Group. This is an Accepted Manuscript of an article published by Taylor & Francis in Biomarkers on 19 April 2018, available online: http://www.tandfonline.com/10.1080/1354750X.2018.1463565