Differential cortical aspartate uptake across the oestrous cycle is associated with changes in gut microbiota in Wistar-Kyoto rats

Simple item page
dc.check.date2025-12-26en
dc.check.infoAccess to this article is restricted until 12 months after publication by request of the publisheren
dc.contributor.authorSajjad, Jahangiren
dc.contributor.authorMorael, Jenniferen
dc.contributor.authorMelo, Thieza G.en
dc.contributor.authorFoley, Taraen
dc.contributor.authorMurphy, Amyen
dc.contributor.authorKeane, Jamesen
dc.contributor.authorPopov, Jelenaen
dc.contributor.authorStanton, Catherineen
dc.contributor.authorDinan, Timothy G.en
dc.contributor.authorClarke, Gerarden
dc.contributor.authorCryan, John F.en
dc.contributor.authorCollins, James M.en
dc.contributor.authorO’Mahony, Siobhain M.en
dc.contributor.funderScience Foundation Irelanden
dc.date.accessioned2025-01-14T09:27:16Z
dc.date.available2025-01-14T09:27:16Z
dc.date.issued2024-12-26en
dc.description.abstractPain and psychological stress are intricately linked, with sex differences evident in disorders associated with both systems. Glutamatergic signalling in the central nervous system is influenced by gonadal hormones via the hypothalamic–pituitary–adrenal axis and is central in pain research. Emerging evidence supports an important role for the gut microbiota in influencing pain signalling. Here, the functional activity of excitatory amino acid transporters (EAATs) in the anterior cingulate cortex (ACC) and lumbosacral spinal cord of male and female Wistar-Kyoto rats, an animal model of comorbid visceral hypersensitivity and enhanced stress responsivity, was investigated across the oestrous cycle. Correlations between the gut microbiota and changes in the functional activity of the central glutamatergic system were also investigated. EAAT function in the lumbosacral spinal cord was similar between males and females across the oestrous cycle. EAAT function was higher in the ACC of dioestrus females compared to proestrus and oestrus females. In males, aspartate uptake in the ACC positively correlated with Bacteroides, while aspartate uptake in the spinal cord positively correlated with the relative abundance of Lachnospiraceae NK4A136. Positive associations with aspartate uptake in the spinal cord were also observed for Alistipes and Bifidobacterium during oestrus, and Eubacterium coprostanoligenes during proestrus. Clostridium sensu stricto1 was negatively associated with aspartate uptake in the ACC in males and dioestrus females. These data indicate that glutamate metabolism in the ACC is oestrous stage-dependent and that short-chain fatty acid-producing bacteria are positively correlated with aspartate uptake in males and during specific oestrous stages in females.en
dc.description.statusPeer revieweden
dc.description.versionAccepted Versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.articleid138096en
dc.identifier.citationSajjad, J., Morael, J., Melo, T. G., Foley, T., Murphy, A., Keane, J., Popov, J., Stanton, C., Dinan, T. G., Clarke, G. and Cryan, J. F. (2024) 'Differential cortical aspartate uptake across the oestrous cycle is associated with changes in gut microbiota in Wistar-Kyoto rats', Neuroscience Letters, 847. 138096 (8pp). https://doi.org/10.1016/j.neulet.2024.138096en
dc.identifier.doihttps://doi.org/10.1016/j.neulet.2024.138096en
dc.identifier.endpage8en
dc.identifier.issn0304-3940en
dc.identifier.journaltitleNeuroscience Lettersen
dc.identifier.startpage1en
dc.identifier.urihttps://hdl.handle.net/10468/16808
dc.identifier.volume847en
dc.language.isoenen
dc.publisherElsevier B.V.en
dc.relation.ispartofNeuroscience Lettersen
dc.relation.projectinfo:eu-repo/grantAgreement/SFI/SFI Research Centres/12/RC/2273/IE/Alimentary Pharmabiotic Centre (APC) - Interfacing Food & Medicine/en
dc.relation.projectinfo:eu-repo/grantAgreement/SFI/SFI Research Centres Programme::Phase 2/12/RC/2273_P2/IE/APC_Phase 2/en
dc.rights© 2024, Elsevier B.V. All rights are reserved, including those for text and data mining, AI training, and similar technologies. This manuscript version is made available under the CC BY-NC-ND 4.0 license.en
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/4.0/en
dc.subjectBrain-gut microbiota axisen
dc.subjectSex differencesen
dc.subjectGlutamateen
dc.subjectSpinal corden
dc.subjectVisceral painen
dc.titleDifferential cortical aspartate uptake across the oestrous cycle is associated with changes in gut microbiota in Wistar-Kyoto ratsen
dc.typeArticle (peer-reviewed)en
oaire.citation.volume847en
Files
Original bundle
Now showing 1 - 2 of 2
Thumbnail Image
Name:
WKY glutamate sex microbiome paper_R1.docx
Size:
1.61 MB
Format:
Microsoft Word XML
Description:
Authors' original accepted version
Thumbnail Image
Name:
WKY glutamate sex microbiome paper_R1.pdf
Size:
1009.7 KB
Format:
Adobe Portable Document Format
Description:
Accepted Version
License bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
license.txt
Size:
2.71 KB
Format:
Item-specific license agreed upon to submission
Description:
Download
Collections
Anatomy and Neuroscience - Journal Articles
APC Microbiome Ireland - Journal Articles
Psychiatry - Journal Articles