Interlaboratory comparison with a reference measurement procedure (RMP) for determining 24,25-dihydroxyvitamin D3 in human serum using liquid chromatography-tandem mass spectrometry (LC-MS/MS)

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dc.contributor.authorWise, Stephen A.
dc.contributor.authorMineva, Ekaterina
dc.contributor.authorPfeiffer, Christine M.
dc.contributor.authorVesper, Hubert
dc.contributor.authorCavalier, Étienne
dc.contributor.authorPeeters, Stéphanie
dc.contributor.authorGalvin, Karen
dc.contributor.authorCashman, Kevin D.
dc.contributor.authorHoofnagle, Andrew N.
dc.contributor.authorWilliams, Emma L.
dc.contributor.authorBriggs, Laura E.
dc.contributor.authorSouness, Megan E.
dc.contributor.authorKuszak, Adam J.
dc.contributor.authorHahm, Grace
dc.contributor.authorCamara, Johanna E.
dc.date.accessioned2026-05-26T15:50:01Z
dc.date.available2026-05-26T15:50:01Z
dc.date.issued2026-04-28
dc.description.abstractAn interlaboratory comparison study was conducted among five laboratories for determining 24,25-dihydroxyvitamin D3 (24,25(OH)2D3) in human serum using liquid chromatography-tandem mass spectrometry (LC-MS/MS) methods. The Centers for Disease Control and Prevention (CDC), Imperial College Healthcare NHS Trust, University College Cork, University of Liège, and University of Washington analyzed 50 single-donor samples and two new Standard Reference Materials (SRMs®). The results from each laboratory were compared with target values assigned by the National Institute of Standards and Technology (NIST) using a reference measurement procedure (RMP) and evaluated using Ordinary Deming linear regression and Bland-Altman analysis. Three of the five laboratory methods provided results that were in good agreement with the NIST RMP results showing linear regression slopes ranging from 0.972 to 1.003 and Bland-Altman mean bias of −0.092 nmol/L, 0.025 nmol/L, and 0.035 nmol/L. Two laboratories demonstrated a significant positive bias with linear regression slopes of 1.158 and 1.214 and Bland-Altman mean bias of 0.162 nmol/L and 0.708 nmol/L. The CDC method is currently used to assign “information only” values for 24,25(OH)2D3 in the quarterly distributions of the Vitamin D External Quality Assessment Scheme (DEQAS). Given the agreement (linear regression slope = 0.984, R2 = 0.996 and mean bias of −0.092 nmol/L) between the CDC method and the NIST RMP observed in this study, the CDC-assigned 24,25(OH)2D3 values in DEQAS may provide a more accurate reference than the current participant consensus mean values.en
dc.description.versionPublished Version
dc.format.extent16
dc.format.mimetypeapplication/pdfen
dc.identifier.authororcidWise, Stephen A.
dc.identifier.authororcidMineva, Ekaterina
dc.identifier.authororcidPfeiffer, Christine M.
dc.identifier.authororcidVesper, Hubert
dc.identifier.authororcidCavalier, Étienne
dc.identifier.authororcidPeeters, Stéphanie
dc.identifier.authororcidGalvin, Karen
dc.identifier.authororcidCashman, Kevin D.
dc.identifier.authororcidHoofnagle, Andrew N.
dc.identifier.authororcidWilliams, Emma L.
dc.identifier.authororcidBriggs, Laura E.
dc.identifier.authororcidSouness, Megan E.
dc.identifier.authororcidKuszak, Adam J.
dc.identifier.authororcidHahm, Grace
dc.identifier.authororcidCamara, Johanna E.
dc.identifier.citationWise, S A, Mineva, E, Pfeiffer, C M, Vesper, H, Cavalier, É, Peeters, S, Galvin, K, Cashman, K D, Hoofnagle, A N, Williams, E L, Briggs, L E, Souness, M E, Kuszak, A J, Hahm, G & Camara, J E 2026, 'Interlaboratory comparison with a reference measurement procedure (RMP) for determining 24,25-dihydroxyvitamin D3 in human serum using liquid chromatography-tandem mass spectrometry (LC-MS/MS)', Analytical and Bioanalytical Chemistry, pp. 1-16. https://doi.org/10.1007/s00216-026-06480-8
dc.identifier.doi10.1007/s00216-026-06480-8
dc.identifier.endpage16
dc.identifier.issn1618-2642
dc.identifier.journaltitleAnalytical and Bioanalytical Chemistry
dc.identifier.otherRIS: urn:948700D960759F4EE34C85D1EDE02FE5
dc.identifier.startpage1
dc.identifier.urihttps://hdl.handle.net/10468/18901
dc.language.isoen
dc.publisherSpringer Science and Business Media Deutschland GmbH
dc.relation.urihttps://www.scopus.com/pages/publications/105037525020
dc.relation.urihttps://www.scopus.com/pages/publications/105037525020?origin=resultslist
dc.rights© 2026. This is a U.S. Government work and not under copyright protection in the US. This is an open access article distributed under the terms of the Creative Commons CC BY license, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
dc.rights.accessrightsopen access
dc.rights.licensenameAttribution 4.0 International
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.statusPeer reviewed
dc.subject24,25-Dihydroxyvitamin D<sub>3</sub>
dc.subject25-Hydroxyvitamin D<sub>2</sub>
dc.subject25-Hydroxyvitamin D<sub>3</sub>
dc.subjectBody fluids
dc.subjectCertified reference materials (CRMs)
dc.subjectDisease control
dc.subjectLaboratories
dc.subjectLinear regression
dc.subjectLiquid chromatography
dc.subjectQuality control
dc.subjectStandard reference materials (SRMs)
dc.subjectVitamin D External Quality Assessment Scheme (DEQAS)
dc.subjectVitamin-D
dc.subjectVitamins
dc.subject[FoodNutritionalSciences]
dc.titleInterlaboratory comparison with a reference measurement procedure (RMP) for determining 24,25-dihydroxyvitamin D3 in human serum using liquid chromatography-tandem mass spectrometry (LC-MS/MS)en
dc.typeArticle (peer-reviewed)
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