Deficiency of essential dietary n-3 PUFA disrupts the caecal microbiome and metabolome in mice

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dc.contributor.authorRobertson, Ruairi C.
dc.contributor.authorSeira Oriach, Clara
dc.contributor.authorMurphy, Kiera
dc.contributor.authorMoloney, Gerard M.
dc.contributor.authorCryan, John F.
dc.contributor.authorDinan, Timothy G.
dc.contributor.authorRoss, R. Paul
dc.contributor.authorStanton, Catherine
dc.contributor.funderScience Foundation Irelanden
dc.contributor.funderHealth Research Boarden
dc.contributor.funderMarine Instituteen
dc.contributor.funderDepartment of Agriculture, Food and the Marineen
dc.contributor.funderEnterprise Irelanden
dc.date.accessioned2018-01-10T16:35:03Z
dc.date.available2018-01-10T16:35:03Z
dc.date.issued2017-11-27
dc.date.updated2018-01-10T15:47:19Z
dc.description.abstractn-3 PUFA are lipids that play crucial roles in immune-regulation, cardio-protection and neurodevelopment. However, little is known about the role that these essential dietary fats play in modulating caecal microbiota composition and the subsequent production of functional metabolites. To investigate this, female C57BL/6 mice were assigned to one of three diets (control (CON), n-3 supplemented (n3+) or n-3 deficient (n3−)) during gestation, following which their male offspring were continued on the same diets for 12 weeks. Caecal content of mothers and offspring were collected for 16S sequencing and metabolic phenotyping. n3− male offspring displayed significantly less % fat mass than n3+ and CON. n-3 Status also induced a number of changes to gut microbiota composition such that n3− offspring had greater abundance of Tenericutes, Anaeroplasma and Coriobacteriaceae. Metabolomics analysis revealed an increase in caecal metabolites involved in energy metabolism in n3+ including α-ketoglutaric acid, malic acid and fumaric acid. n3− animals displayed significantly reduced acetate, butyrate and total caecal SCFA production. These results demonstrate that dietary n-3 PUFA regulate gut microbiota homoeostasis whereby n-3 deficiency may induce a state of disturbance. Further studies are warranted to examine whether these microbial and metabolic disturbances are causally related to changes in metabolic health outcomes.en
dc.description.sponsorshipHealth Research Board, Ireland (grant nos HRA_POR/2011/23 and HRA_POR/2012/32);en
dc.description.statusPeer revieweden
dc.description.versionPublished Versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.citationRobertson, R. C., Seira Oriach, C., Murphy, K., Moloney, G. M., Cryan, J. F., Dinan, T. G., Ross, R. P. and Stanton, C. (2017) 'Deficiency of essential dietary n-3 PUFA disrupts the caecal microbiome and metabolome in mice', British Journal of Nutrition, 118(11), pp. 959-970. doi: 10.1017/S0007114517002999en
dc.identifier.doi10.1017/S0007114517002999
dc.identifier.endpage970en
dc.identifier.issn0007-1145
dc.identifier.issued11en
dc.identifier.journaltitleBritish Journal of Nutritionen
dc.identifier.startpage959en
dc.identifier.urihttps://hdl.handle.net/10468/5267
dc.identifier.volume118en
dc.language.isoenen
dc.publisherCambridge University Press (CUP)en
dc.relation.projectinfo:eu-repo/grantAgreement/SFI/SFI Research Centres/12/RC/2273/IE/Alimentary Pharmabiotic Centre (APC) - Interfacing Food & Medicine/en
dc.relation.urihttps://www.cambridge.org/core/article/deficiency-of-essential-dietary-n3-pufa-disrupts-the-caecal-microbiome-and-metabolome-in-mice/A8BCA9EB0AC0E9F67737796EF5FAA871
dc.rights© The Authors 2017en
dc.subjectMicrobiomeen
dc.subjectMicrobiotaen
dc.subjectn-3 PUFAen
dc.subjectSCFAen
dc.subjectMetabolomicsen
dc.titleDeficiency of essential dietary n-3 PUFA disrupts the caecal microbiome and metabolome in miceen
dc.typeArticle (peer-reviewed)en
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