Antiproliferation activity and mechanism of c9, t11, c15-CLNA and t9, t11, c15-CLNA from Lactobacillus plantarum ZS2058 on colon cancer cells

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dc.contributor.authorRen, Qing
dc.contributor.authorYang, Bo
dc.contributor.authorZhu, Guangzhen
dc.contributor.authorWang, Shunyu
dc.contributor.authorFu, Chengli
dc.contributor.authorZhang, Hao
dc.contributor.authorRoss, R. Paul
dc.contributor.authorStanton, Catherine
dc.contributor.authorChen, Haiqin
dc.contributor.authorChen, Wei
dc.contributor.funderNational Natural Science Foundation of Chinaen
dc.contributor.funderFundamental Research Funds for the Central Universitiesen
dc.contributor.funderNational First-Class Discipline Program of Food Science and Technologyen
dc.contributor.funderCollaborative Innovationcenter of Food Safety and Quality Control in Jiangsu Provinceen
dc.date.accessioned2023-03-15T14:40:35Z
dc.date.available2023-03-15T14:40:35Z
dc.date.issued2020-03
dc.date.updated2023-03-15T12:45:07Z
dc.description.abstractConjugated linolenic acid (CLNA) is a type of ω-3 fatty acid which has been proven to have a series of benefits. However, there is no study about the function of Lactobacillus-derived CLNA isomer. Lactobacillus plantarum ZS2058 has been proven to manifest comprehensive functions and can produce CLNA. To investigate the specific functions of CLNA produced by this probiotic bacterium, two different conjugated a-linolenic acid (CLNA) isomers were successfully isolated. These isoforms, CLNA1 (c9, t11, c15-CLNA, purity 97.48%) and CLNA2 (c9, t11, t15-CLNA, purity 99.00%), both showed the ability to inhibit the growth of three types of colon cancer cells in a time- and concentration-dependent manner. In addition, the expression of MDA in Caco-2 cells was increased by CLNA1 or CLNA2, which indicated that lipid peroxidation was related to the antiproliferation activity of CLNAs. An examination of the key protein of pyroptosis showed that CLNA1 induced the cleavage of caspase-1 and gasdermin-D, while CLNA2 induced the cleavage of caspase-4, 5 and gasdermin-D. The addition of relative inhibitors could alleviate the pyroptosis by CLNAs. CLNA1 and CLNA2 showed no effect on caspase-3, 7, 9 and PARP-1, which were key proteins associated with apoptosis. No sub-diploid apoptotic peak appeared in the result of PI single staining test. In conclusion, CLNA1 activated caspase-1 and induced Caco-2 cell pyroptosis, whereas CLNA2 induced pyroptosis through the caspase-4/5-mediated pathway. The inhibition of Caco-2 cells by the two isomers was not related to apoptosis. This is the first study on the function of Lactobacillus-derived CLNA isomer. The inhibition pathway of Lactobacillus-derived CLNA isomer on colon cancer cells were proved.en
dc.description.sponsorshipNational Natural Science Foundation of China (Nos. 31722041, 31801521, 31530056); Fundamental Research Funds for the Central Universities (Nos. JUSRP51702A, JUSRP11733); National First-Class Discipline Program of Food Science and Technology (No. JUFSTR20180102); Jangsu Province “Collaborative Innovation Center for Food Safety and Quality Control”, the Postgraduate Research and Practice Innovation Program of Jiangsu Province (KYCX18_1763)en
dc.description.statusPeer revieweden
dc.description.versionPublished Versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.articleid1225en
dc.identifier.citationRen, Q., Yang, B., Zhu, G., Wang, S., Fu, C., Zhang, H., Ross, R.P., Stanton, C., Chen, H. and Chen, W. (2020) ‘Antiproliferation activity and mechanism of c9, t11, c15-clna and t9, t11, c15-CLNA from Lactobacillus plantarum zs2058 on colon cancer cells’, Molecules, 25(5), 1225 (17pp). doi: 10.3390/molecules25051225en
dc.identifier.doi10.3390/molecules25051225en
dc.identifier.endpage17en
dc.identifier.issn1420-3049
dc.identifier.issued5en
dc.identifier.journaltitleMolecules (Basel, Switzerland)en
dc.identifier.startpage1en
dc.identifier.urihttps://hdl.handle.net/10468/14311
dc.identifier.volume25en
dc.language.isoenen
dc.publisherMDPI AGen
dc.rights© 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).en
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/en
dc.subjectApoptosisen
dc.subjectCaco-2 cellen
dc.subjectConjugated linolenic aciden
dc.subjectLipid peroxidationen
dc.subjectPyroptosisen
dc.titleAntiproliferation activity and mechanism of c9, t11, c15-CLNA and t9, t11, c15-CLNA from Lactobacillus plantarum ZS2058 on colon cancer cellsen
dc.typeArticle (peer-reviewed)en
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