Induction of broad immunity by thermostabilised vaccines incorporated in dissolvable microneedles using novel fabrication methods

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dc.contributor.authorVrdoljak, Anto
dc.contributor.authorAllen, Evin A.
dc.contributor.authorFerrara, Francesca
dc.contributor.authorTemperton, Nigel J.
dc.contributor.authorCrean, Abina M.
dc.contributor.authorMoore, Anne C.
dc.contributor.funderScience Foundation Irelanden
dc.contributor.funderEnterprise Irelanden
dc.contributor.funderUniversity College Corken
dc.date.accessioned2019-08-21T09:01:23Z
dc.date.available2019-08-21T09:01:23Z
dc.date.issued2016-03
dc.date.updated2019-08-21T09:00:46Z
dc.description.abstractDissolvable microneedle (DMN) patches for immunization have multiple benefits, including vaccine stability and ease-of-use. However, conventional DMN fabrication methods have several drawbacks. Here we describe a novel, microfluidic, drop dispensing-based dissolvable microneedle production method that overcomes these issues. Uniquely, heterogeneous arrays, consisting of microneedles of diverse composition, can be easily produced on the same patch. Robustness of the process was demonstrated by incorporating and stabilizing adenovirus and MVA vaccines. Clinically-available trivalent inactivated influenza vaccine (TIV) in DMN patches is fully stable for greater than 6months at 40 degrees C. Immunization using low dose TIV-loaded DMN patches induced significantly higher antibody responses compared to intramuscular-based immunization in mice. TIV-loaded patches also induced a broader, heterosubtypic neutralizing antibody response. By addressing issues that will be faced in large-scale fill-finish DMN fabrication processes and demonstrating superior thermostable characteristics and immunogenicity, this study progresses the translation of this microneedle platform to eventual clinical deployment.en
dc.description.sponsorshipScience Foundation Ireland (SFI Grant NAP280); Enterprise Ireland (Grant CF2011 1634); University College Cork (Studentship, Colleeg of Medicine and Health, UCC)en
dc.description.statusPeer reviewed
dc.description.versionAccepted Version
dc.identifier.citationVrdoljak, A., Allen, E. A., Ferrara, F., Temperton, N. J., Crean, A. M. and Moore, A. C. (2016) 'Induction of broad immunity by thermostabilised vaccines incorporated in dissolvable microneedles using novel fabrication methods', Journal of Controlled Release, 225, pp. 192-204. doi: 10.1016/j.jconrel.2016.01.019en
dc.identifier.doi10.1016/j.jconrel.2016.01.019
dc.identifier.endpage204
dc.identifier.issn1873-4995
dc.identifier.journaltitleJournal of Controlled Releaseen
dc.identifier.startpage192
dc.identifier.urihttps://hdl.handle.net/10468/8362
dc.identifier.volume225
dc.language.isoenen
dc.publisherElsevieren
dc.relation.urihttps://www.sciencedirect.com/science/article/pii/S0168365916300189
dc.rights© 2016 Elsevier B.V. All rights reserved. This manuscript version is made available under the CC BY-NC-ND 4.0 licence.en
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/4.0/en
dc.subjectBroadly-neutralizing antibody
dc.subjectInfluenza vaccine
dc.subjectMicroneedle
dc.subjectStability
dc.subjectVirus vector vaccine
dc.titleInduction of broad immunity by thermostabilised vaccines incorporated in dissolvable microneedles using novel fabrication methodsen
dc.typeArticle (peer-reviewed)en
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