Hypermethylation of MAPK13 promoter in oesophageal squamous cell carcinoma Is associated with loss of p38δ MAPK expression

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dc.contributor.authorO'Callaghan, Carol
dc.contributor.authorFanning, Liam J.
dc.contributor.authorBarry, Orla P.
dc.contributor.funderHealth Research Boarden
dc.date.accessioned2016-03-09T13:59:33Z
dc.date.available2016-03-09T13:59:33Z
dc.date.issued2015-10-23
dc.date.updated2015-11-04T10:54:31Z
dc.description.abstractThe loss of tumour suppressor gene function is a hallmark of malignant transformation and can occur by a variety of genetic and/or epigenetic alterations. We have previously characterised p38δ mitogen-activated protein kinase (MAPK) as a tumour suppressor in oesophageal squamous cell carcinoma (OESCC) and outlined how loss of p38δ MAPK expression promotes increased proliferation and migration, as well as reduced chemosensitivity. Our aim was to investigate the underlying molecular causes of loss of p38δ MAPK expression in OESCC. Sequence analysis of DNA from p38δ MAPK positive and p38δ MAPK negative OESCC cell lines was used to investigate potential loss of function causing mutations. Epigenetic control of p38δ expression in OESCC was examined using methylation-specific PCR and sequencing of bisulfite-converted DNA. We did not identify any mutations in the MAPK13 sequence in OESCC cell lines which lack p38δ MAPK expression. However, we identified a differential pattern of methylation between p38δ MAPK positive and p38δ MAPK negative cell lines. We outline here for the first time differential MAPK13 promoter methylation in OESCC. Our results suggest that epigenetic alterations are responsible, in part, for the suppression of p38δ MAPK expression and promotion of tumourigenesis in OESCC.en
dc.description.sponsorshipHealth Research Board, Ireland (Grant HRA/2009/17)en
dc.description.statusPeer revieweden
dc.description.versionPublished Versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.citationO CALLAGHAN, C., FANNING, L. & BARRY, O. 2015. Hypermethylation of MAPK13 Promoter in Oesophageal Squamous Cell Carcinoma Is Associated with Loss of p38δ MAPK Expression. Cancers, 7, 0881. http://www.mdpi.com/2072-6694/7/4/0881en
dc.identifier.doi10.3390/cancers7040881
dc.identifier.endpage2133en
dc.identifier.issn2072-6694
dc.identifier.issued4en
dc.identifier.journaltitleCancersen
dc.identifier.startpage2124en
dc.identifier.urihttps://hdl.handle.net/10468/2426
dc.identifier.volume7en
dc.language.isoenen
dc.publisherMDPI AGen
dc.rights© 2015 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons by Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).en
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.subjectEpigeneticsen
dc.subjectMethylationen
dc.subjectOesophageal squamous cell carcinomaen
dc.subjectp38δen
dc.subjectMAPKen
dc.subjectMitogen activated protein kinase 13en
dc.subjectp38delta MAPKen
dc.subjectMessenger RNAen
dc.subjectDNAen
dc.titleHypermethylation of MAPK13 promoter in oesophageal squamous cell carcinoma Is associated with loss of p38δ MAPK expressionen
dc.typeArticle (peer-reviewed)en
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