Immunomodulatory activity of 5 kDa permeate fractions of casein hydrolysates generated using a range of enzymes in Jurkat T cells and RAW264.7 macrophages
dc.contributor.author | O'Sullivan, Siobhán M. | |
dc.contributor.author | O'Callaghan, Yvonne C. | |
dc.contributor.author | O'Keeffe, Martina B. | |
dc.contributor.author | FitzGerald, Richard J. | |
dc.contributor.author | O'Brien, Nora M. | |
dc.contributor.funder | Food Institutional Research Measure | en |
dc.contributor.funder | Department of Agriculture, Food and the Marine | en |
dc.date.accessioned | 2019-01-17T11:49:27Z | |
dc.date.available | 2019-01-17T11:49:27Z | |
dc.date.issued | 2018-12-31 | |
dc.date.updated | 2019-01-17T11:43:47Z | |
dc.description.abstract | The in vitro bioactivity of 5 kDa ultrafiltration permeate fractions of casein hydrolysates produced using different enzymes were compared. Reverse phase ultra-performance liquid chromatography and gel permeation chromatography showed that the permeates had different physicochemical properties (molecular mass and degree of hydrolysis). The Flavourzyme® permeate had the highest activity in the 2,2’-azinobis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) assay. Cellular antioxidant and immunomodulatory assays showed that none of the permeates exhibited in vitro antioxidant activity, while all permeates significantly (P < 0.05) decreased interleukin-6 (IL-6) production in ConA-stimulated Jurkat T cells at 0.50% (w/v) and LPS-stimulated RAW264.7 cells at 0.05 and 0.50% (w/v). Three permeates, obtained using Flavourzyme®, Flavorpro Whey and trypsin, also significantly (P < 0.05) decreased IL-1β production at 0.05% (w/v) in RAW264.7 cells. Western blot analysis showed that all permeates significantly decreased the expression of the NF-κB subunit, p65, in RAW264.7 cells indicating that anti-inflammatory activity may be associated with this pathway. | en |
dc.description.sponsorship | Irish Department of Agriculture, Food and the Marine (DAFM and the Food Institutional Research Measure (FIRM), both funded by the Irish Government under the National Development Plan 2007-2013 under grant issue 11F063) | en |
dc.description.status | Peer reviewed | en |
dc.description.version | Accepted Version | en |
dc.format.mimetype | application/pdf | en |
dc.identifier.citation | O’Sullivan, S. M., O’Callaghan, Y. C., O’Keeffe, M. B., FitzGerald, R. J. and O’Brien, N. M. (2018) 'Immunomodulatory activity of 5 kDa permeate fractions of casein hydrolysates generated using a range of enzymes in Jurkat T cells and RAW264.7 macrophages', International Dairy Journal, In Press, doi: 10.1016/j.idairyj.2018.12.005 | en |
dc.identifier.doi | 10.1016/j.idairyj.2018.12.005 | |
dc.identifier.endpage | 38 | en |
dc.identifier.issn | 0958-6946 | |
dc.identifier.journaltitle | International Dairy Journal | en |
dc.identifier.startpage | 1 | en |
dc.identifier.uri | https://hdl.handle.net/10468/7308 | |
dc.language.iso | en | en |
dc.publisher | Elsevier | en |
dc.relation.uri | http://www.sciencedirect.com/science/article/pii/S0958694618302760 | |
dc.rights | © 2018 Elsevier Ltd. All rights reserved. This manuscript version is made available under the CC-BY-NC-ND 4.0 license | en |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | en |
dc.subject | Casein | en |
dc.subject | Bioactivity | en |
dc.subject | Protein hydrolysates | en |
dc.subject | Anti-inflammatory activity | en |
dc.subject | In vitro bioactivity | en |
dc.subject | Casein hydrolysates | en |
dc.title | Immunomodulatory activity of 5 kDa permeate fractions of casein hydrolysates generated using a range of enzymes in Jurkat T cells and RAW264.7 macrophages | en |
dc.type | Article (peer-reviewed) | en |
Files
Original bundle
1 - 1 of 1
Loading...
- Name:
- 8865_IDJ_accepted_manuscript.pdf
- Size:
- 1014.31 KB
- Format:
- Adobe Portable Document Format
- Description:
- Published version
License bundle
1 - 1 of 1
Loading...
- Name:
- license.txt
- Size:
- 2.71 KB
- Format:
- Item-specific license agreed upon to submission
- Description: