Immunomodulatory activity of 5 kDa permeate fractions of casein hydrolysates generated using a range of enzymes in Jurkat T cells and RAW264.7 macrophages

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dc.contributor.authorO'Sullivan, Siobhán M.
dc.contributor.authorO'Callaghan, Yvonne C.
dc.contributor.authorO'Keeffe, Martina B.
dc.contributor.authorFitzGerald, Richard J.
dc.contributor.authorO'Brien, Nora M.
dc.contributor.funderFood Institutional Research Measureen
dc.contributor.funderDepartment of Agriculture, Food and the Marineen
dc.date.accessioned2019-01-17T11:49:27Z
dc.date.available2019-01-17T11:49:27Z
dc.date.issued2018-12-31
dc.date.updated2019-01-17T11:43:47Z
dc.description.abstractThe in vitro bioactivity of 5 kDa ultrafiltration permeate fractions of casein hydrolysates produced using different enzymes were compared. Reverse phase ultra-performance liquid chromatography and gel permeation chromatography showed that the permeates had different physicochemical properties (molecular mass and degree of hydrolysis). The Flavourzyme® permeate had the highest activity in the 2,2’-azinobis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) assay. Cellular antioxidant and immunomodulatory assays showed that none of the permeates exhibited in vitro antioxidant activity, while all permeates significantly (P < 0.05) decreased interleukin-6 (IL-6) production in ConA-stimulated Jurkat T cells at 0.50% (w/v) and LPS-stimulated RAW264.7 cells at 0.05 and 0.50% (w/v). Three permeates, obtained using Flavourzyme®, Flavorpro Whey and trypsin, also significantly (P < 0.05) decreased IL-1β production at 0.05% (w/v) in RAW264.7 cells. Western blot analysis showed that all permeates significantly decreased the expression of the NF-κB subunit, p65, in RAW264.7 cells indicating that anti-inflammatory activity may be associated with this pathway.en
dc.description.sponsorshipIrish Department of Agriculture, Food and the Marine (DAFM and the Food Institutional Research Measure (FIRM), both funded by the Irish Government under the National Development Plan 2007-2013 under grant issue 11F063)en
dc.description.statusPeer revieweden
dc.description.versionAccepted Versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.citationO’Sullivan, S. M., O’Callaghan, Y. C., O’Keeffe, M. B., FitzGerald, R. J. and O’Brien, N. M. (2018) 'Immunomodulatory activity of 5 kDa permeate fractions of casein hydrolysates generated using a range of enzymes in Jurkat T cells and RAW264.7 macrophages', International Dairy Journal, In Press, doi: 10.1016/j.idairyj.2018.12.005en
dc.identifier.doi10.1016/j.idairyj.2018.12.005
dc.identifier.endpage38en
dc.identifier.issn0958-6946
dc.identifier.journaltitleInternational Dairy Journalen
dc.identifier.startpage1en
dc.identifier.urihttps://hdl.handle.net/10468/7308
dc.language.isoenen
dc.publisherElsevieren
dc.relation.urihttp://www.sciencedirect.com/science/article/pii/S0958694618302760
dc.rights© 2018 Elsevier Ltd. All rights reserved. This manuscript version is made available under the CC-BY-NC-ND 4.0 licenseen
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/en
dc.subjectCaseinen
dc.subjectBioactivityen
dc.subjectProtein hydrolysatesen
dc.subjectAnti-inflammatory activityen
dc.subjectIn vitro bioactivityen
dc.subjectCasein hydrolysatesen
dc.titleImmunomodulatory activity of 5 kDa permeate fractions of casein hydrolysates generated using a range of enzymes in Jurkat T cells and RAW264.7 macrophagesen
dc.typeArticle (peer-reviewed)en
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