A new macro-imager based on Tpx3Cam optical camera for PLIM applications

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dc.contributor.authorSen, Rajannya
dc.contributor.authorZhdanov, Alexander V.
dc.contributor.authorHirvonen, Liisa
dc.contributor.authorSvihra, Peter
dc.contributor.authorAndersson-Engels, Stefan
dc.contributor.authorNomerotski, Andrei
dc.contributor.authorPapkovsky, Dmitri B.
dc.contributor.funderScience Foundation Irelanden
dc.date.accessioned2021-08-26T10:03:50Z
dc.date.available2021-08-26T10:03:50Z
dc.date.issued2020-04-01
dc.date.updated2021-08-26T09:34:35Z
dc.description.abstractThe recently designed Tpx3Cam camera based PLIM (Phosphorescence Lifetime IMaging) macro-imager was tested using an array of phosphorescent chemical and biological samples. A series of sensor materials prepared by incorporating the phosphorescent O2-sensitive dye, PtBP, into five polymers with different O2 permeability were imaged along with several commercial and non-commercial sensors based on PtBP and PtOEPK dyes. The PLIM images showed good lifetime contrast between the different materials, and phosphorescence lifetime values obtained were consistent with those measured by alternative methods. A panel of live tissues samples stained with PtBP based nanoparticle probe were also prepared and imaged under resting conditions and upon inhibition of respiration. The macro-imager showed promising results as a tool for PLIM of O2 in chemical and biological samples.en
dc.description.sponsorshipScience Foundation Ireland (SFI/12/RC/2276_P2 and SFI/17/RC-PhD/3484)en
dc.description.statusPeer revieweden
dc.description.versionPublished Versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.articleid113591Nen
dc.identifier.citationSen, R., Zhdanov, A., Hirvonen, L., Svihra, P., Andersson-Engels, S., Nomerotski, A. and Papkovsky, D. (2020) ‘A new macro-imager based on Tpx3Cam optical camera for PLIM applications’, Proceedings of SPIE, April, 113591N (9 pp) doi: 10.1117/12.2555387en
dc.identifier.doi10.1117/12.2555387en
dc.identifier.endpage8en
dc.identifier.issn1996-756X
dc.identifier.startpage1en
dc.identifier.urihttps://hdl.handle.net/10468/11791
dc.language.isoenen
dc.publisherSociety of Photo-Optical Instrumentation Engineers (SPIE)en
dc.relation.projectinfo:eu-repo/grantAgreement/SFI/SFI Research Centres/12/RC/2276/IE/I-PIC Irish Photonic Integration Research Centre/en
dc.rights© 2020 Society of Photo-Optical Instrumentation Engineers (SPIE). One print or electronic copy may be made for personal use only. Systematic reproduction and distribution, duplication of any material in this paper for a fee or for commercial purposes, or modification of the content of the paper are prohibited.en
dc.subjectFluorescence and Phosphorescence Lifetime Imaging (FLIM and PLIM)en
dc.subjectOxygen sensing and imagingen
dc.subjectTime Correlated Single Photon Counting (TCSPC)en
dc.subjectTpx3Cam cameraen
dc.subjectSensorsen
dc.subjectImaging systemsen
dc.subjectCamerasen
dc.subjectFluorescence lifetime imagingen
dc.subjectPhotographyen
dc.subjectSingle photonen
dc.subjectPhosphorescenceen
dc.titleA new macro-imager based on Tpx3Cam optical camera for PLIM applicationsen
dc.typeConference itemen
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