A new macro-imager based on Tpx3Cam optical camera for PLIM applications
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Date
2020-04-01
Authors
Sen, Rajannya
Zhdanov, Alexander V.
Hirvonen, Liisa
Svihra, Peter
Andersson-Engels, Stefan
Nomerotski, Andrei
Papkovsky, Dmitri B.
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Publisher
Society of Photo-Optical Instrumentation Engineers (SPIE)
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Abstract
The recently designed Tpx3Cam camera based PLIM (Phosphorescence Lifetime IMaging) macro-imager was tested using an array of phosphorescent chemical and biological samples. A series of sensor materials prepared by incorporating the phosphorescent O2-sensitive dye, PtBP, into five polymers with different O2 permeability were imaged along with several commercial and non-commercial sensors based on PtBP and PtOEPK dyes. The PLIM images showed good lifetime contrast between the different materials, and phosphorescence lifetime values obtained were consistent with those measured by alternative methods. A panel of live tissues samples stained with PtBP based nanoparticle probe were also prepared and imaged under resting conditions and upon inhibition of respiration. The macro-imager showed promising results as a tool for PLIM of O2 in chemical and biological samples.
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Keywords
Fluorescence and Phosphorescence Lifetime Imaging (FLIM and PLIM) , Oxygen sensing and imaging , Time Correlated Single Photon Counting (TCSPC) , Tpx3Cam camera , Sensors , Imaging systems , Cameras , Fluorescence lifetime imaging , Photography , Single photon , Phosphorescence
Citation
Sen, R., Zhdanov, A., Hirvonen, L., Svihra, P., Andersson-Engels, S., Nomerotski, A. and Papkovsky, D. (2020) ‘A new macro-imager based on Tpx3Cam optical camera for PLIM applications’, Proceedings of SPIE, April, 113591N (9 pp) doi: 10.1117/12.2555387
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© 2020 Society of Photo-Optical Instrumentation Engineers (SPIE). One print or electronic copy may be made for personal use only. Systematic reproduction and distribution, duplication of any material in this paper for a fee or for commercial purposes, or modification of the content of the paper are prohibited.