Silver citrate nanoparticles inhibit PMA-induced TNF alpha expression via deactivation of NF-kappa B activity in human cancer cell-lines, MCF-7

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dc.contributor.authorAbdellatif, Ahmed A. H.
dc.contributor.authorRasheed, Zafar
dc.contributor.authorAlhowail, Ahmad H.
dc.contributor.authorAlqasoumi, Abdulmajeed
dc.contributor.authorAlsharidah, Mansour
dc.contributor.authorKhan, Riaz A.
dc.contributor.authorAljohani, Abdullah S. M.
dc.contributor.authorAldubayan, Maha A.
dc.contributor.authorFaisal, Waleed
dc.contributor.funderQassim Universityen
dc.date.accessioned2021-02-11T12:23:10Z
dc.date.available2021-02-11T12:23:10Z
dc.date.issued2020-10-30
dc.date.updated2021-02-09T11:47:56Z
dc.description.abstractBackground: The nuclear factor kappa-B (NF-κB) is a major transcription factor responsible for the production of numerous inflammatory mediators, including the tumor necrosis factor (TNFα), which has a lethal association with cancer’s onset. The silver nanoparticles (AgNPs) are widely used in cancer treatment and several other biomedical applications. Objective: The study aimed to determine the effects of silver citrate nanoparticles (AgNPs-CIT) on NF-κB activation together with TNFα mRNA/protein expressions in the phorbol myristate acetate (PMA)-stimulated MCF-7 human breast cancer cell-lines. Methods: The AgNPs-CIT were synthesized by the reduction method, and the prepared AgNPs-CIT were characterized for their shape, absorption in UV-VIS electromagnetic radiations, size distribution, ζ-potential, and antioxidant activity. The MCF-7 cell-lines were pretreated with AgNPs-CIT and stimulated with PMA. The TNFα mRNA expressions were determined by real-time PCR, whereas the protein production was determined by the ELISA. The NF-κB activity was distinctly observed by highly-specific DNA-based ELISA, and by NF-κB-specific inhibitor, Bay 11– 7082. Results: The prepared AgNPs-CIT were spherical and have an absorption wavelength range of 381– 452 nm wherein the particles size ranged between 19.2± 0.1 to 220.77± 0.12 nm with the charge range − 9.99± 0.8 to − 34.63± 0.1 mV. The prepared AgNPs-CIT showed comparative antioxidant activity at > 40% inhibitions level of the DPPH radicals. The AgNPs-CIT were found to be non-toxic to MCF-7 cell-lines and inhibited PMA-induced activation of the NF-κBp65, and also the mRNA/protein expression of TNFα. Conclusion: This is the first report that showed AgNPs-CIT inhibited TNFα expression via deactivation of the NF-κB signaling event in stimulated breast cancer cells. The results have important implications for the development of novel therapeutic strategies for the prevention/treatment of cancers and/or inflammatory disorders.en
dc.description.sponsorshipQassim University (represented by the Deanship of Scientific Research, under the number (5551-pharmacy-2019-2-2-I) during the academic years 1440 AH/2019 AD)en
dc.description.statusPeer revieweden
dc.description.versionPublished Versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.citationAbdellatif, A. A. H., Rasheed, Z., Alhowail, A. H., Alqasoum.i A., Alsharidah, M., Khan, R. A., Aljohani, A. S. M, Aldubayan, M. A., and Faisal,W. (2020) 'Silver Citrate Nanoparticles Inhibit PMA-Induced TNFα Expression via Deactivation of NF-κB Activity in Human Cancer Cell-Lines, MCF-7', International Journal of Nanomedicine, 15, pp. 8479-8493. doi: 10.2147/IJN.S274098en
dc.identifier.doi10.2147/IJN.S274098en
dc.identifier.endpage8493en
dc.identifier.issn1178-2013
dc.identifier.journaltitleInternational Journal of Nanomedicineen
dc.identifier.startpage8479en
dc.identifier.urihttps://hdl.handle.net/10468/11060
dc.identifier.volume15en
dc.language.isoenen
dc.publisherDove Medical Pressen
dc.rights© 2020 Abdellatif et al. This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution - Non Commercial (unported, v3.0) License. By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms.en
dc.rights.urihttps://creativecommons.org/licenses/by-nc/3.0/en
dc.subjectPhorbol myristate acetateen
dc.subjectPMAen
dc.subjectTNFαen
dc.subjectNF-κBen
dc.subjectSilver citrate nanoparticlesen
dc.subjectMCF-7 cell linesen
dc.subjectCanceren
dc.subjectInflammatory cytokinesen
dc.subjectNecrosis-factor-alphaen
dc.subjectGold nanoparticlesen
dc.subjectCyclooxygenase-2en
dc.subjectTranscriptionen
dc.subjectSomatostatinen
dc.subjectMetaanalysisen
dc.subjectAggregationen
dc.subjectAntioxidanten
dc.titleSilver citrate nanoparticles inhibit PMA-induced TNF alpha expression via deactivation of NF-kappa B activity in human cancer cell-lines, MCF-7en
dc.typeArticle (peer-reviewed)en
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