A bioengineered nisin derivative, M21A, in combination with food grade additives eradicates biofilms of Listeria monocytogenes

dc.contributor.authorSmith, Muireann K.
dc.contributor.authorDraper, Lorraine A.
dc.contributor.authorHazelhoff, Pieter-Jan
dc.contributor.authorCotter, Paul D.
dc.contributor.authorRoss, R. Paul
dc.contributor.authorHill, Colin
dc.contributor.funderScience Foundation Irelanden
dc.date.accessioned2016-12-14T14:22:03Z
dc.date.available2016-12-14T14:22:03Z
dc.date.issued2016-11-30
dc.date.updated2016-12-14T14:13:42Z
dc.description.abstractThe burden of foodborne disease has large economic and social consequences worldwide. Despite strict regulations, a number of pathogens persist within the food environment, which is greatly contributed to by a build-up of resistance mechanisms and also through the formation of biofilms. Biofilms have been shown to be highly resistant to a number of antimicrobials and can be extremely difficult to remove once they are established. In parallel, the growing concern of consumers regarding the use of chemically derived antimicrobials within food has led to a drive toward more natural products. As a consequence, the use of naturally derived antimicrobials has become of particular interest. In this study we investigated the efficacy of nisin A and its bioengineered derivative M21A in combination with food grade additives to treat biofilms of a representative foodborne disease isolate of Listeria monocytogenes. Investigations revealed the enhanced antimicrobial effects, in liquid culture, of M21A in combination with citric acid or cinnamaldehyde over its wild type nisin A counterpart. Subsequently, an investigation was conducted into the effects of these combinations on an established biofilm of the same strain. Nisin M21A (0.1 μg/ml) alone or in combination with cinnamaldehyde (35 μg/ml) or citric acid (175 μg/ml) performed significantly better than combinations involving nisin A. All combinations of M21A with either citric acid or cinnamaldehyde eradicated the L. monocytogenes biofilm (in relation to a non-biofilm control). We conclude that M21A in combination with available food additives could further enhance the antimicrobial treatment of biofilms within the food industry, simply by substituting nisin A with M21A in current commercial products such as Nisaplin® (Danisco, DuPont).en
dc.description.sponsorshipScience Foundation Ireland (Grant Number SFI/12/RC/2273)en
dc.description.statusPeer revieweden
dc.description.versionPublished Versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.citationSmith, M. K., Draper, L. A., Hazelhoff, P.-J., Cotter, P. D., Ross, R. P. and Hill, C. (2016) 'A Bioengineered Nisin Derivative, M21A, in Combination with Food Grade Additives Eradicates Biofilms of Listeria monocytogenes', Frontiers in Microbiology, 7(1939). doi:10.3389/fmicb.2016.01939en
dc.identifier.doi10.3389/fmicb.2016.01939
dc.identifier.endpage1939-11en
dc.identifier.issn1664-302X
dc.identifier.journaltitleFrontiers In Microbiologyen
dc.identifier.startpage1939-1en
dc.identifier.urihttps://hdl.handle.net/10468/3380
dc.identifier.volume7en
dc.language.isoenen
dc.publisherFrontiers Mediaen
dc.rights© 2016 Smith, Draper, Hazelhoff, Cotter, Ross and Hill. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.en
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/en
dc.subjectListeria monocytogenesen
dc.subjectBiofilmen
dc.subjectNisinen
dc.subjectBioengineereden
dc.subjectCitric aciden
dc.subjectCinnamaldehydeen
dc.subjectCombinationsen
dc.titleA bioengineered nisin derivative, M21A, in combination with food grade additives eradicates biofilms of Listeria monocytogenesen
dc.typeArticle (peer-reviewed)en
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