dc.contributor.author	Lawson, Melissa A. E.
dc.contributor.author	O'Neill, Ian J.
dc.contributor.author	Kujawska, Magdalena
dc.contributor.author	Gowrinadh Javvadi, Sree
dc.contributor.author	Wijeyesekera, Anisha
dc.contributor.author	Flegg, Zak
dc.contributor.author	Chalklen, Lisa
dc.contributor.author	Hall, Lindsay J.
dc.contributor.funder	Wellcome Trust	en
dc.contributor.funder	Biotechnology and Biological Sciences Research Council	en
dc.contributor.funder	Institute Strategic Programme Gut Microbes and Health	en
dc.contributor.funder	Institute Strategic Programme Gut Health and Food Safety	en
dc.contributor.funder	Marie Sklodowska-Curie Individual Fellowship	en
dc.date.accessioned	2019-12-09T10:58:17Z
dc.date.available	2019-12-09T10:58:17Z
dc.date.issued	2019-11-18
dc.description.abstract	Diet-microbe interactions play an important role in modulating the early-life microbiota, with Bifidobacterium strains and species dominating the gut of breast-fed infants. Here, we sought to explore how infant diet drives distinct bifidobacterial community composition and dynamics within individual infant ecosystems. Genomic characterisation of 19 strains isolated from breast-fed infants revealed a diverse genomic architecture enriched in carbohydrate metabolism genes, which was distinct to each strain, but collectively formed a pangenome across infants. Presence of gene clusters implicated in digestion of human milk oligosaccharides (HMOs) varied between species, with growth studies indicating that within single infants there were differences in the ability to utilise 2′FL and LNnT HMOs between strains. Cross-feeding experiments were performed with HMO degraders and non-HMO users (using spent or ‘conditioned’ media and direct co-culture). Further 1H-NMR analysis identified fucose, galactose, acetate, and N-acetylglucosamine as key by-products of HMO metabolism; as demonstrated by modest growth of non-HMO users on spend media from HMO metabolism. These experiments indicate how HMO metabolism permits the sharing of resources to maximise nutrient consumption from the diet and highlights the cooperative nature of bifidobacterial strains and their role as ‘foundation’ species in the infant ecosystem. The intra- and inter-infant bifidobacterial community behaviour may contribute to the diversity and dominance of Bifidobacterium in early life and suggests avenues for future development of new diet and microbiota-based therapies to promote infant health.	en
dc.description.sponsorship	Wellcome Trust Investigator Award (100/974/C/13/Z); BBSRC Norwich Research Park Bioscience Doctoral Training Grant (BB/M011216/1); Institute Strategic Programme Gut Microbes and Health (BB/R012490/1, and its constituent project(s) BBS/E/F/000PR10353 and BBS/E/F/000PR10356); nstitute Strategic Programme Gut Health and Food Safety (BB/J004529/1);	en
dc.description.status	Peer reviewed	en
dc.description.version	Published Version	en
dc.format.mimetype	application/pdf	en
dc.identifier.citation	Lawson, M. A. E., O’Neill, I. J., Kujawska, M., Gowrinadh Javvadi, S., Wijeyesekera, A., Flegg, Z., Chalklen, L. and Hall, L. J. (2019) 'Breast milk-derived human milk oligosaccharides promote Bifidobacterium interactions within a single ecosystem', The ISME Journal. (14pp.) doi: 10.1038/s41396-019-0553-2	en
dc.identifier.doi	10.1038/s41396-019-0553-2	en
dc.identifier.eissn	1751-7370
dc.identifier.endpage	14	en
dc.identifier.issn	1751-7362
dc.identifier.journaltitle	The ISME Journal	en
dc.identifier.startpage	1	en
dc.identifier.uri	https://hdl.handle.net/10468/9357
dc.language.iso	en	en
dc.publisher	Springer Nature	en
dc.relation.project	info:eu-repo/grantAgreement/EC/H2020::MSCA-IF-EF-ST/661594/EU/Elucidating how Bifidobacteria shapes the microbiota in response to infant diet./INFANT MICROBIOTA	en
dc.rights	© The Author(s) 2019. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.	en
dc.rights.uri	http://creativecommons.org/licenses/by/4.0/	en
dc.subject	Bacterial physiology	en
dc.subject	Microbial ecology	en
dc.subject	Microbiome	en
dc.title	Breast milk-derived human milk oligosaccharides promote Bifidobacterium interactions within a single ecosystem	en
dc.type	Article (peer-reviewed)	en

Breast milk-derived human milk oligosaccharides promote Bifidobacterium interactions within a single ecosystem

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