Reincarnation of bacteriocins From the Lactobacillus pangenomic graveyard

Simple item page
dc.contributor.authorCollins, Fergus W. J.
dc.contributor.authorMesa-Pereira, Beatriz
dc.contributor.authorO'Connor, Paula M.
dc.contributor.authorRea, Mary C.
dc.contributor.authorHill, Colin
dc.contributor.authorRoss, R. Paul
dc.contributor.funderScience Foundation Ireland
dc.date.accessioned2018-08-29T15:47:27Z
dc.date.available2018-08-29T15:47:27Z
dc.date.issued2018
dc.description.abstractBacteria commonly produce narrow spectrum bacteriocins as a means of inhibiting closely related species competing for similar resources in an environment. The increasing availability of genomic data means that it is becoming easier to identify bacteriocins encoded within genomes. Often, however, the presence of bacteriocin genes in a strain does not always translate into biological antimicrobial activity. For example, when analysing the Lactobacillus pangenome we identified strains encoding ten pediocin-like bacteriocin structural genes which failed to display inhibitory activity. Nine of these bacteriocins were novel whilst one was identified as the previously characterized bacteriocin "penocin A." The composition of these bacteriocin operons varied between strains, often with key componentsmissing which are required for bacteriocin production, such as dedicated bacteriocin transporters and accessory proteins. In an effort to functionally express these bacteriocins, the structural genes for the ten pediocin homologs were cloned alongside the dedicated pediocin PA-1 transporter in both Escherichia coli and Lactobacillus paracasei heterologous hosts. Each bacteriocin was cloned with its native leader sequence and as a fusion protein with the pediocin PA-1 leader sequence. Several of these bacteriocins displayed a broader spectrum of inhibition than the original pediocin PA-1. We show how potentially valuable bacteriocins can easily be "reincarnated" from in silico data and produced in vitro despite often lacking the necessary accompanying machinery. Moreover, the study demonstrates how genomic datasets such as the Lactobacilus pangenome harbor a potential "arsenal" of antimicrobial activity with the possibility of being activated when expressed in more genetically amenable hosts.en
dc.description.sponsorshipScience Foundation Ireland (SFI/12/RC/227)en
dc.description.statusPeer revieweden
dc.description.versionPublished Versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.articleid1298
dc.identifier.citationCollins, F. W. J., Mesa-Pereira, B., O'Connor, P. M., Rea, M. C., Hill, C. and Ross, R. P. (2018) 'Reincarnation of Bacteriocins From the Lactobacillus Pangenomic Graveyard', Frontiers in Microbiology, 9, 1298 (9pp). doi: 10.3389/fmicb.2018.01298en
dc.identifier.doi10.3389/fmicb.2018.01298
dc.identifier.endpage9
dc.identifier.issn1664-302X
dc.identifier.journaltitleFrontiers in Microbiologyen
dc.identifier.startpage1
dc.identifier.urihttps://hdl.handle.net/10468/6675
dc.identifier.volume9
dc.language.isoenen
dc.publisherFrontiers Mediaen
dc.relation.urihttps://www.frontiersin.org/articles/10.3389/fmicb.2018.01298/full
dc.rights© 2018, Collins, Mesa Pereira, O'Connor, Rea, Hill and Ross. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.en
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjectBacteriocinsen
dc.subjectPediocinen
dc.subjectHeterologous expressionen
dc.subjectEscherichia colien
dc.subjectLactobacillusen
dc.titleReincarnation of bacteriocins From the Lactobacillus pangenomic graveyarden
dc.typeArticle (peer-reviewed)en
Files
Original bundle
Now showing 1 - 2 of 2
Thumbnail Image
Name:
Reincarnation of Bacteriocins From the Lactobacillus.pdf
Size:
584.44 KB
Format:
Adobe Portable Document Format
Description:
Published Version
Download
Thumbnail Image
Name:
Reincarnation of Bacteriocins From the Lactobacillus-1.docx
Size:
290.93 KB
Format:
Microsoft Word XML
Description:
Supplemental File 1
Download
Collections
APC Microbiome Ireland - Journal Articles
Microbiology - Journal Articles