Ethanolamine is a novel STAT-3 dependent cardioprotective agent

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dc.contributor.authorKelly-Laubscher, Roisin
dc.contributor.authorLamont, Kim T.
dc.contributor.authorSomers, Sarin
dc.contributor.authorHacking, Damian
dc.contributor.authorLacerda, Lydia
dc.contributor.authorThomas, Paul
dc.contributor.authorOpie, Lionel H.
dc.contributor.authorLecour, Sandrine
dc.contributor.funderNational Research Foundationen
dc.contributor.funderSouth African Medical Research Councilen
dc.contributor.funderClaude Leon Foundationen
dc.date.accessioned2022-01-26T14:56:52Z
dc.date.available2022-01-26T14:56:52Z
dc.date.issued2010-10-12
dc.date.updated2022-01-26T14:42:32Z
dc.description.abstractEthanolamine is a biogenic amine found naturally in the body as part of membrane lipids and as a metabolite of the cardioprotective substances, sphingosine-1-phosphate (S1P) and anandamide. In the brain, ethanolamine, formed from the breakdown of anandamide protects against ischaemic apoptosis. However, the effects of ethanolamine in the heart are unknown. Signal transducer and activator of transcription 3 (STAT-3) is a critical prosurvival factor in ischaemia/reperfusion (I/R) injury. Therefore, we investigated whether ethanolamine protects the heart via activation of STAT-3. Isolated hearts from wildtype or cardiomyocyte specific STAT-3 knockout (K/O) mice were pre-treated with ethanolamine (Etn) (0.3 mmol/L) before I/R insult. In vivo rat hearts were subjected to 30 min ischaemia/2 h reperfusion in the presence or absence of 5 mg/kg S1P and/or the FAAH inhibitor, URB597. Infarct size was measured at the end of each protocol by triphenyltetrazolium chloride staining. Pre-treatment with ethanolamine decreased infarct size in isolated mouse or rat hearts subjected to I/R but this infarct sparing effect was lost in cardiomyocyte specific STAT-3 deficient mice. Pre-treatment with ethanolamine increased nuclear phosphorylated STAT-3 [control 0.75 ± 0.08 vs. Etn 1.50 ± 0.09 arbitrary units; P < 0.05]. Our findings suggest a novel cardioprotective role for ethanolamine against I/R injury via activation of STAT-3.en
dc.description.sponsorshipNational Research Foundation of South Africa, NRF; South African Medical Research Counci (Inter-University Cape Heart Group of the South African Medical Research Council and the Servier Heart Failure Project); Claude Leon Foundationen
dc.description.statusPeer revieweden
dc.description.versionAccepted Versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.citationKelly, R. F., Lamont, K. T., Somers, S., Hacking, D., Lacerda, L., Thomas, P., Opie, L. H. and Lecour, S. (2010) 'Ethanolamine is a novel STAT-3 dependent cardioprotective agent', Basic Research in Cardiology, 105 (6), pp. 763-770. doi: 10.1007/s00395-010-0125-0en
dc.identifier.doi10.1007/s00395-010-0125-0en
dc.identifier.eissn1435-1803
dc.identifier.endpage770en
dc.identifier.issn0300-8428
dc.identifier.issued6en
dc.identifier.journaltitleBasic Research in Cardiologyen
dc.identifier.startpage763en
dc.identifier.urihttps://hdl.handle.net/10468/12482
dc.identifier.volume105en
dc.language.isoenen
dc.publisherSpringeren
dc.rights© Springer-Verlag 2010. This is a post-peer-review, pre-copyedit version of an article published in Basic Research in Cardiology. The final authenticated version is available online at: http://dx.doi.org/10.1007/s00395-010-0125-0en
dc.rights.urihttps://link.springer.com/article/10.1007/s12012-015-9355-6en
dc.subjectEthanolamineen
dc.subjectSphingosine-1-phosphateen
dc.subjectIschaemia-reperfusionen
dc.subjectCardioprotectionen
dc.subjectSTAT-3en
dc.titleEthanolamine is a novel STAT-3 dependent cardioprotective agenten
dc.typeArticle (peer-reviewed)en
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