Effects of endurance racing on horse plasma extracellular particle miRNA

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Date
2020-06-02
Authors
de Oliveira Jr, Getúlio P.
Porto, William F.
Palu, Cintia C.
Pereira, Lydyane M.
Reis, Alessandra M. M.
Marçola, Tatiana G.
Teixeira-Neto, Antonio R.
Franco, Octavio L.
Pereira, Rinaldo W.
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John Wiley & Sons, Inc.
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Abstract
Background: Physical exercise is an essential factor in preventing and treating metabolic diseases by promoting systemic benefits throughout the body. The molecular factors involved in this process are poorly understood. Micro RNAs (miRNAs) are small non-coding RNAs that inhibit mRNA transcription. MiRNAs, which can participate in the benefits of exercise to health, circulate in plasma in extracellular particles (EP). Horses that undergo endurance racing are an excellent model to study the impact of long-duration/low intensity exercise in plasma EP miRNAs. Objectives: To evaluate the effects of 160 km endurance racing on horse plasma extracellular particles and their miRNA population. Study design: Cohort study. Methods: We collected plasma from five Arabian horses during five time-points of an endurance ride. Extracellular particles were purified from plasma and characterised by electron microscopy, resistive pulse sensing (qNano) and western blotting. Small RNAs were purified from horse plasma EP, and sequencing was performed. Results: Endurance racing increased EP concentration and average diameter compared to before the race. Western blotting showed a high concentration of extracellular vesicles proteins 2 hours after the race, which returned to baseline 15 hours after the race. MicroRNA differential expression analysis revealed increasing levels of eca-miR-486-5p during and after the race, and decreasing levels of eca-miR-9083 after the end. Conclusions: This study adds new data about the variation in plasma EP concentrations after long-distance exercise and brings new insights about the roles of exercise-derived EP miRNAs during low-intensity endurance exercise.
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Keywords
Horse , Endurance racing , Extracellular particles , EP , MicroRNA , miRNA
Citation
de Oliveira, G. P., Porto, W. F., Palu, C.C., Pereira, L. M., Reis, A. M. M., Marçola, T. G., Teixeira-Neto, A. R., Franco, O. L. and Pereira, R. W. (2021) 'Effects of endurance racing on horse plasma extracellular particle miRNA', Equine Veterinary Journal, 53(3), pp. 618-627. doi: 10.1111/evj.13300
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© 2020, Equine Veterinary Journal. Published by John Wiley & Sons, Inc. This is the peer reviewed version of the following item: de Oliveira, G. P., Porto, W. F., Palu, C.C., Pereira, L. M., Reis, A. M. M., Marçola, T. G., Teixeira-Neto, A. R., Franco, O. L. and Pereira, R. W. (2021) 'Effects of endurance racing on horse plasma extracellular particle miRNA', Equine Veterinary Journal, 53(3), pp. 618-627, doi: 10.1111/evj.13300, which has been published in final form at: https://doi.org/10.1111/evj.13300 This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions.