Genomic characterization of Listeria monocytogenes isolates associated with clinical listeriosis and the food production environment in Ireland

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dc.contributor.authorHilliard, Amber
dc.contributor.authorLeong, Dara
dc.contributor.authorO'Callaghan, Amy
dc.contributor.authorCulligan, Eamonn P.
dc.contributor.authorMorgan, Ciara
dc.contributor.authorDeLappe, Niall
dc.contributor.authorHill, Colin
dc.contributor.authorJordan, Kieran
dc.contributor.authorCormican, Martin
dc.contributor.authorGahan, Cormac G.
dc.contributor.funderScience Foundation Ireland
dc.contributor.funderDepartment of Agriculture, Food and the Marine
dc.date.accessioned2018-05-02T10:16:28Z
dc.date.available2018-05-02T10:16:28Z
dc.date.issued2018
dc.description.abstractListeria monocytogenes is a major human foodborne pathogen that is prevalent in the natural environment and has a high case fatality rate. Whole genome sequencing (WGS) analysis has emerged as a valuable methodology for the classification of L. monocytogenes isolates and the identification of virulence islands that may influence infectivity. In this study, WGS was used to provide an insight into 25 L. monocytogenes isolates from cases of clinical infection in Ireland between 2013 and 2015. Clinical strains were either lineage I (14 isolates) or lineage II (11 isolates), with 12 clonal complexes (CC) represented, of which CC1 (6) and CC101 (4) were the most common. Single nucleotide polymorphism (SNP) analysis demonstrated that clinical isolates from mother–infant pairs (one isolate from the mother and one from the infant) were highly related (3 SNP differences in each) and also identified close similarities between isolates from otherwise distinct cases (1 SNP difference). Clinical strains were positive for common virulence-associated loci and 13 isolates harbour the LIPI-3 locus. Pulsed-field gel electrophoresis (PFGE) was used to compare strains to a database of 1300 Irish food and food processing environment isolates and determined that 64% of clinical pulsotypes were previously encountered in the food or food processing environment. Five of the matching food and food processing environment isolates were sequenced and results demonstrated a correlation between pulsotype and genotype. Overall, the work provides insights into the nature of L. monocytogenes strains currently causing clinical disease in Ireland and indicates that similar isolates can be found in the food or food processing environment.en
dc.description.sponsorshipDepartment of Agriculture, Food and the Marine (Food Institutional Research Measure (FIRM) 11F008)en
dc.description.statusPeer revieweden
dc.description.versionPublished Versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.articleid171
dc.identifier.citationHilliard, A., Leong, D., O’Callaghan, A., Culligan, E., Morgan, C., DeLappe, N., Hill, C., Jordan, K., Cormican, M. and Gahan, C. (2018) 'Genomic characterization of Listeria monocytogenes isolates associated with clinical listeriosis and the food production environment in Ireland', Genes, 9(3), 171 (16pp). doi: 10.3390/genes9030171en
dc.identifier.doi10.3390/genes9030171
dc.identifier.endpage16
dc.identifier.issn2073-4425
dc.identifier.issued3
dc.identifier.journaltitleGenesen
dc.identifier.startpage1
dc.identifier.urihttps://hdl.handle.net/10468/5955
dc.identifier.volume9
dc.language.isoenen
dc.publisherMDPI AGen
dc.relation.projectinfo:eu-repo/grantAgreement/SFI/SFI Research Centres/12/RC/2273/IE/Alimentary Pharmabiotic Centre (APC) - Interfacing Food & Medicine/
dc.relation.urihttp://www.mdpi.com/2073-4425/9/3/171
dc.rights© 2018, the Authors. Licensee MDPI, Basel, Switzerland. This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).en
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjectListeria monocytogenesen
dc.subjectClinicalen
dc.subjectGenomeen
dc.subjectGenomicsen
dc.subjectSequenceen
dc.subjectSingle nucleotide polymorphismen
dc.subjectSNPen
dc.titleGenomic characterization of Listeria monocytogenes isolates associated with clinical listeriosis and the food production environment in Irelanden
dc.typeArticle (peer-reviewed)en
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