dc.contributor.author	Kavanaugh, Devon W.
dc.contributor.author	O'Callaghan, John
dc.contributor.author	Butto, Ludovica F.
dc.contributor.author	Slattery, Helen
dc.contributor.author	Lane, Jonathan
dc.contributor.author	Clyne, Marguerite
dc.contributor.author	Kane, Marian
dc.contributor.author	Joshi, Lokesh
dc.contributor.author	Hickey, Rita M.
dc.contributor.funder	Science Foundation Ireland	en
dc.contributor.funder	Teagasc	en
dc.date.accessioned	2016-02-17T11:45:33Z
dc.date.available	2016-02-17T11:45:33Z
dc.date.issued	2013
dc.description.abstract	In this study, we tested the hypothesis that milk oligosaccharides may contribute not only to selective growth of bifidobacteria, but also to their specific adhesive ability. Human milk oligosaccharides (3'sialyllactose and 6'sialyllactose) and a commercial prebiotic (Beneo Orafti P95; oligofructose) were assayed for their ability to promote adhesion of Bifidobacterium longum subsp. infantis ATCC 15697 to HT-29 and Caco-2 human intestinal cells. Treatment with the commercial prebiotic or 3'sialyllactose did not enhance adhesion. However, treatment with 6'sialyllactose resulted in increased adhesion (4.7 fold), while treatment with a mixture of 3'- and 6'-sialyllactose substantially increased adhesion (9.8 fold) to HT-29 intestinal cells. Microarray analyses were subsequently employed to investigate the transcriptional response of B. longum subsp. infantis to the different oligosaccharide treatments. This data correlated strongly with the observed changes in adhesion to HT-29 cells. The combination of 3'- and 6'-sialyllactose resulted in the greatest response at the genetic level (both in diversity and magnitude) followed by 6'sialyllactose, and 3'sialyllactose alone. The microarray data was further validated by means of real-time PCR. The current findings suggest that the increased adherence phenotype of Bifidobacterium longum subsp. infantis resulting from exposure to milk oligosaccharides is multi-faceted, involving transcription factors, chaperone proteins, adhesion-related proteins, and a glycoside hydrolase. This study gives additional insight into the role of milk oligosaccharides within the human intestine and the molecular mechanisms underpinning host-microbe interactions.	en
dc.description.sponsorship	Science Foundation Ireland (SFI Grant No. 08/SRC/B1393, Alimentary Glycoscience Research Cluster (AGRC)); Teagasc (Walsh Fellowship).	en
dc.description.status	Peer reviewed	en
dc.description.version	Published Version	en
dc.format.mimetype	application/pdf	en
dc.identifier.articleid	e67224
dc.identifier.citation	Kavanaugh DW, O’Callaghan J, Buttó LF, Slattery H, Lane J, Clyne M, et al. (2013) Exposure of Bifidobacterium longum subsp. infantis to Milk Oligosaccharides Increases Adhesion to Epithelial Cells and Induces a Substantial Transcriptional Response. PLoS ONE 8(6): e67224. doi:10.1371/journal.pone.0067224	en
dc.identifier.doi	10.1371/journal.pone.0067224
dc.identifier.issn	1932-6203
dc.identifier.issued	6	en
dc.identifier.journaltitle	PLOS ONE	en
dc.identifier.uri	https://hdl.handle.net/10468/2373
dc.identifier.volume	8	en
dc.language.iso	en	en
dc.publisher	Public Library of Science	en
dc.rights	© 2013 Kavanaugh et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited	en
dc.rights.uri	http://creativecommons.org/licenses/by/4.0/	en
dc.subject	Heat shock protein	en
dc.subject	Bottle fed infants	en
dc.subject	In-vitro	en
dc.subject	Helicobacter pylori	en
dc.subject	Caco-2 cells	en
dc.subject	Intestinal microflora	en
dc.subject	Haemophilus ducreyi	en
dc.subject	Bacterial adhesion	en
dc.subject	Human enterocyte	en
dc.subject	Strains	en
dc.title	Exposure of Bifidobacterium longum subsp infantis to milk oligosaccharides increases adhesion to epithelial cells and induces a substantial transcriptional response	en
dc.type	Article (peer-reviewed)	en

Exposure of Bifidobacterium longum subsp infantis to milk oligosaccharides increases adhesion to epithelial cells and induces a substantial transcriptional response

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