The human mesenteric lymph node microbiome differentiates between Crohn’s disease and ulcerative colitis

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dc.contributor.authorMcDermott, Kieran
dc.contributor.authorKiernan, Miranda G.
dc.contributor.authorKiely, Patrick A.
dc.contributor.authorDunne, Colum P.
dc.contributor.authorCoffey, J. Calvin
dc.contributor.authorCotter, Paul D.
dc.contributor.authorCabrera-Rubio, Raul
dc.contributor.funderUniversity of Limericken
dc.date.accessioned2019-05-15T05:11:02Z
dc.date.available2019-05-15T05:11:02Z
dc.date.issued2018-09-15
dc.description.abstractMesenteric lymph nodes are sites in which translocated bacteria incite and progress immunological responses. For this reason, understanding the microbiome of mesenteric lymph nodes in inflammatory bowel disease is important. The bacterial profile of Crohn’s disease mesenteric lymph nodes has been analysed using culture-independent methods in only one previous study. This study aimed to investigate the mesenteric lymph node microbiota from both Crohn’s disease and ulcerative colitis patients.Mesenteric lymph nodes were collected from Crohn’s disease and ulcerative colitis patients undergoing resection. Total DNA was extracted from mesenteric lymph nodes and assessed for the presence of bacterial DNA [16S]. All work was completed in a sterile environment using aseptic techniques. Samples positive for 16S DNA underwent next-generation sequencing, and the identity of bacterial phyla and species were determined.Crohn’s disease mesenteric lymph nodes had a distinctly different microbial profile to that observed in ulcerative colitis. The relative abundance of Firmicutes was greater in nodes from ulcerative colitis patients, whereas Proteobacteria were more abundant in Crohn’s disease. Although species diversity was reduced in the mesenteric lymph nodes of patients with Crohn’s disease, these lymph nodes contained greater numbers of less dominant phyla, mainly Fusobacteria.This study confirms that there are distinct differences between the Crohn’s disease and ulcerative colitis mesenteric lymph node microbiomes. Such microbial differences could aid in the diagnosis of Crohn’s disease or ulcerative colitis, particularly in cases of indeterminate colitis at time of resection, or help explain their mechanisms of development and progression.en
dc.description.sponsorshipUniversity of Limerick (Graduate Entry Medical School Strategic Research Fund)en
dc.description.statusPeer revieweden
dc.description.versionPublished Versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.citationKiernan, M.G., Coffey, J.C., McDermott, K., Cotter, P.D., Cabrera-Rubio, R., Kiely, P.A. and Dunne, C.P., 2018. The human mesenteric lymph node microbiome differentiates between Crohn’s disease and ulcerative colitis. Journal of Crohn's and Colitis, 13(1), pp. 58-66. DOI: 10.1093/ecco-jcc/jjy136en
dc.identifier.doi10.1093/ecco-jcc/jjy136en
dc.identifier.endpage66en
dc.identifier.issn1873-9946
dc.identifier.issued1en
dc.identifier.journaltitleJournal of Crohn's and Colitisen
dc.identifier.startpage58en
dc.identifier.urihttps://hdl.handle.net/10468/7905
dc.identifier.volume13en
dc.language.isoenen
dc.publisherOxford University Pressen
dc.relation.urihttps://academic.oup.com/ecco-jcc/article/13/1/58/5098279
dc.rights© European Crohn’s and Colitis Organisation (ECCO) 2018. This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/ licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited.en
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/en
dc.subjectInflammatory bowel diseaseen
dc.subjectMicrobiomeen
dc.subjectMesenteric lymph nodesen
dc.titleThe human mesenteric lymph node microbiome differentiates between Crohn’s disease and ulcerative colitisen
dc.typeArticle (peer-reviewed)en
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