dc.contributor.author	Buttimer, Colin
dc.contributor.author	Hendrix, Hanne
dc.contributor.author	Lucid, Alan
dc.contributor.author	Neve, Horst
dc.contributor.author	Noben, Jean-Paul
dc.contributor.author	Franz, Charles M. A. P.
dc.contributor.author	O'Mahony, Jim
dc.contributor.author	Lavigne, Rob
dc.contributor.author	Coffey, Aidan
dc.contributor.funder	Science Foundation Ireland
dc.contributor.funder	KU Leuven
dc.date.accessioned	2018-09-27T12:08:10Z
dc.date.available	2018-09-27T12:08:10Z
dc.date.issued	2018
dc.description.abstract	Pectobacterium atrosepticum is an economically important phytopathogen that is responsible for potato blackleg and soft rot, and for which current control strategies are limited. In this study, stem samples of potato crops exhibiting blackleg were taken from three farms in Co. Cork, Ireland, and they were found to be infected with P. atrosepticum. Three closely related bacteriophages (phages) that are specific to this phytopathogen were isolated and characterized, namely vB_PatP_CB1, vB_PatP_CB3, and vB_PatP_CB4 (abbreviated as CB1, CB3, and CB4). Both CB1 and CB3 were determined to infect 12 strains and CB4 10 strains of the 19 strains of P. atrosepticum tested. Morphology, latent periods, burst sizes, and their stability at various temperatures and pHs were also examined. Genome sequencing of the three phages revealed that they shared a minimum nucleotide identity of 93% with each other. Their genomes exhibited an Enquartavirinae genome organization, possessing several conserved proteins that were associated with phages of this group, like the type species Escherichia virus N4. Tandem electrospray ionization-mass spectrometry (ESI-MS/MS) allowed for the identification of ten structural proteins that form the virion of CB1, six that are conserved in phage N4. Biocontrol experiments demonstrated that the phages suppress soft rot formation upon co-inoculation with P. atrosepticum on whole tubers. The results of this study indicate that CB1 related phages could be good candidates for phage-based control.	en
dc.description.sponsorship	KU Leuven (GOA project ‘Phage Biosystems' )	en
dc.description.status	Peer reviewed	en
dc.description.version	Published Version	en
dc.format.mimetype	application/pdf	en
dc.identifier.articleid	45
dc.identifier.citation	Buttimer, C., Hendrix, H., Lucid, A., Neve, H., Noben, J.-P., Franz, C., O’Mahony, J., Lavigne, R. and Coffey, A. (2018) 'Novel N4-like bacteriophages of pectobacterium atrosepticum', Pharmaceuticals, 11(2), 45 (22pp). doi: 10.3390/ph11020045	en
dc.identifier.doi	10.3390/ph11020045
dc.identifier.endpage	22
dc.identifier.issn	1424-8247
dc.identifier.issued	2
dc.identifier.journaltitle	Pharmaceuticals	en
dc.identifier.startpage	1
dc.identifier.uri	https://hdl.handle.net/10468/6919
dc.identifier.volume	11
dc.language.iso	en	en
dc.publisher	MDPI AG	en
dc.relation.project	info:eu-repo/grantAgreement/SFI/SFI Research Infrastructure Programme/12/RI/2335 (2)/IE/ICP-HRMS mass spectrometer/
dc.relation.uri	http://www.mdpi.com/1424-8247/11/2/45
dc.rights	© 2018, the Authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).	en
dc.rights.uri	http://creativecommons.org/licenses/by/4.0/
dc.subject	Phage isolation	en
dc.subject	Phage characterization	en
dc.subject	Pectobacterium	en
dc.subject	Blackleg	en
dc.subject	Soft rot	en
dc.subject	Phage-mediated biocontrol of bacteria	en
dc.subject	Plant disease biocontrol	en
dc.subject	Phage therapy	en
dc.subject	N4-like phage	en
dc.title	Novel N4-like bacteriophages of pectobacterium atrosepticum	en
dc.type	Article (peer-reviewed)	en

Novel N4-like bacteriophages of pectobacterium atrosepticum

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