Isolation and characterization of Septuagintavirus ; a novel clade of Escherichia coli phages within the subfamily Vequintavirinae

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dc.contributor.authorCortés-Martín, Adriánen
dc.contributor.authorButtimer, Colinen
dc.contributor.authorPozhydaieva, Nadiiaen
dc.contributor.authorHille, Franken
dc.contributor.authorShareefdeen, Hibaen
dc.contributor.authorBolocan, Andrei Sorinen
dc.contributor.authorDraper, Lorraine A.en
dc.contributor.authorShkoporov, Andrey N.en
dc.contributor.authorFranz, Charles M. A. P.en
dc.contributor.authorHöfer, Katharinaen
dc.contributor.authorRoss, R. Paulen
dc.contributor.authorHill, Colinen
dc.contributor.funderScience Foundation Irelanden
dc.contributor.funderJanssen Biotechen
dc.contributor.funderMax Planck Societyen
dc.contributor.funderDeutsche Forschungsgemeinschaften
dc.date.accessioned2025-03-10T12:10:59Z
dc.date.available2025-03-10T12:10:59Z
dc.date.issued2024en
dc.description.abstractEscherichia coli is a commensal inhabitant of the mammalian gut microbiota, frequently associated with various gastrointestinal diseases. There is increasing interest in comprehending the variety of bacteriophages (phages) that target this bacterium, as such insights could pave the way for their potential use in therapeutic applications. Here, we report the isolation and characterization of four newly identified E. coli infecting tailed phages (W70, A7-1, A5-4, and A73) that were found to constitute a novel genus, Septuagintavirus, within the subfamily Vequintavirinae. Genomes of these phages ranged from 137 kbp to 145 kbp, with a GC content of 41 mol%. They possess a maximum nucleotide similarity of 30% with phages of the closest phylogenetic genus, Certrevirus, while displaying limited homology to other genera of the Vequintavirinae family. Host range analysis showed that these phages have limited activity against a panel of E. coli strains, infecting 6 out of 16 tested isolates, regardless of their phylotype. Electrospray ionization-tandem mass spectrometry (ESI-MS/MS) was performed on the virion of phage W70, allowing the identification of 28 structural proteins, 19 of which were shared with phages of other genera of Vequintavirinae family. The greatest diversity was identified with proteins forming tail fiber structures, likely indicating the adaptation of virions of each phage genus of this subfamily for the recognition of their target receptor on host cells. The findings of this study provide greater insights into the phages of the subfamily Vequintavirinae, contributing to the pool of knowledge currently known about these phages.en
dc.description.statusPeer revieweden
dc.description.versionPublished Versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.articleide00592-24en
dc.identifier.citationCortés-Martín, A., Buttimer, C., Pozhydaieva, N., Hille, F., Shareefdeen, H., Bolocan, A.S., Draper, L.A., Shkoporov, A.N., Franz, C.M.A.P., Höfer, K., Ross, R.P. and Hill, C. (2024) ‘Isolation and characterization of Septuagintavirus ; a novel clade of Escherichia coli phages within the subfamily Vequintavirinae’, Microbiology Spectrum,12(9), e00592-24 (16pp). https://doi.org/10.1128/spectrum.00592-24en
dc.identifier.doi10.1128/spectrum.00592-24en
dc.identifier.eissn2165-0497
dc.identifier.endpage16en
dc.identifier.issued9en
dc.identifier.journaltitleMicrobiology Spectrumen
dc.identifier.startpage1en
dc.identifier.urihttps://hdl.handle.net/10468/17158
dc.identifier.volume12en
dc.language.isoenen
dc.publisherAmerican Society for Microbiologyen
dc.relation.projectinfo:eu-repo/grantAgreement/SFI/SFI Research Centres/12/RC/2273_P2/IE/Alimentary Pharmabiotic Centre (APC) - Interfacing Food & Medicine/en
dc.relation.projectinfo:eu-repo/grantAgreement/SFI/SFI Spokes Programme/14/SP APC/B3032/IE/Gut Phageomics - Phage as diagnostics and/or therapeutics in IBD/en
dc.relation.projectDeutsche Forschungsgemeinschaft (German Research Council DFG SPP 2330, Project number 464500427)en
dc.rights© 2024, Cortés-Martín et al. This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.en
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/en
dc.subjectIsolationen
dc.subjectCharacterizationen
dc.subjectBacteriophageen
dc.subjectEscherichia colien
dc.subjectVequintavirinaeen
dc.subjectVirionen
dc.subjectMass-spectrometren
dc.titleIsolation and characterization of Septuagintavirus ; a novel clade of Escherichia coli phages within the subfamily Vequintavirinaeen
dc.typeArticle (peer-reviewed)en
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