Comparative genome analysis of the Lactobacillus brevis species

Simple item page
dc.contributor.authorFeyereisen, Marine
dc.contributor.authorMahony, Jennifer
dc.contributor.authorKelleher, Philip
dc.contributor.authorRoberts, Richard John
dc.contributor.authorO'Sullivan, Tadhg
dc.contributor.authorGeertman, Jan-Maarten A.
dc.contributor.authorvan Sinderen, Douwe
dc.contributor.funderIrish Research Councilen
dc.contributor.funderScience Foundation Irelanden
dc.date.accessioned2019-11-20T05:26:23Z
dc.date.available2019-11-20T05:26:23Z
dc.date.issued2019-05-23
dc.description.abstractBackground: Lactobacillus brevis is a member of the lactic acid bacteria (LAB), and strains of L. brevis have been isolated from silage, as well as from fermented cabbage and other fermented foods. However, this bacterium is also commonly associated with bacterial spoilage of beer. Results: In the current study, complete genome sequences of six isolated L. brevis strains were determined. Five of these L. brevis strains were isolated from beer (three isolates) or the brewing environment (two isolates), and were characterized as beer-spoilers or non-beer spoilers, respectively, while the sixth isolate had previously been isolated from silage. The genomic features of 19 L. brevis strains, encompassing the six L. brevis strains described in this study and thirteen L. brevis strains for which complete genome sequences were available in public databases, were analyzed with particular attention to evolutionary aspects and adaptation to beer. Conclusions: Comparative genomic analysis highlighted evolution of the taxon allowing niche colonization, notably adaptation to the beer environment, with approximately 50 chromosomal genes acquired by L. brevis beer-spoiler strains representing approximately 2% of their total chromosomal genetic content. These genes primarily encode proteins that are putatively involved in oxidation-reduction reactions, transcription regulation or membrane transport, functions that may be crucial to survive the harsh conditions associated with beer. The study emphasized the role of plasmids in beer spoilage with a number of unique genes identified among L. brevis beer-spoiler strains.en
dc.description.sponsorshipEPSPG/2015/7; 450 13/IA/1953en
dc.description.statusPeer revieweden
dc.description.versionPublished Versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.articleid416en
dc.identifier.citationFeyereisen, M., Mahony, J., Kelleher, P., Roberts, R.J., O’Sullivan, T., Geertman, J.M.A. and van Sinderen, D., 2019. Comparative genome analysis of the Lactobacillus brevis species. BMC genomics, 20(1), (416). DOI:10.1186/s12864-019-5783-1en
dc.identifier.doi10.1186/s12864-019-5783-1en
dc.identifier.eissn1471-2164
dc.identifier.endpage15en
dc.identifier.issued1en
dc.identifier.journaltitleBMC Genomicsen
dc.identifier.startpage1en
dc.identifier.urihttps://hdl.handle.net/10468/9126
dc.identifier.volume20en
dc.language.isoenen
dc.publisherBioMed Centralen
dc.relation.projectinfo:eu-repo/grantAgreement/SFI/SFI Starting Investigator Research Grant (SIRG)/15/SIRG/3430/IE/Phage-host interactome of the dairy bacterium Streptococcus thermophilus (PHIST)/en
dc.relation.urihttps://bmcgenomics.biomedcentral.com/articles/10.1186/s12864-019-5783-1
dc.rights© The Author(s). 2019en
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stateden
dc.subjectLactobacillus brevisen
dc.subjectSMRT sequencingen
dc.subjectGenomicsen
dc.subjectPan-genomeen
dc.subjectBeer adaptationen
dc.subjectBeer spoilageen
dc.titleComparative genome analysis of the Lactobacillus brevis speciesen
dc.typeArticle (peer-reviewed)en
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
s12864-019-5783-1.pdf
Size:
1.37 MB
Format:
Adobe Portable Document Format
Description:
Published version
Download
License bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
license.txt
Size:
2.71 KB
Format:
Item-specific license agreed upon to submission
Description:
Download
Collections
APC Microbiome Ireland - Journal Articles
Microbiology - Journal Articles