dc.contributor.author	Flemer, Burkhardt
dc.contributor.author	Lynch, Denise B.
dc.contributor.author	Brown, Jillian R. M.
dc.contributor.author	Jeffery, Ian B.
dc.contributor.author	Ryan, Feargal J.
dc.contributor.author	Claesson, Marcus J.
dc.contributor.author	O'Riordain, Micheal G.
dc.contributor.author	Shanahan, Fergus
dc.contributor.author	O'Toole, Paul W.
dc.contributor.funder	Science Foundation Ireland
dc.date.accessioned	2018-02-06T13:36:27Z
dc.date.available	2018-02-06T13:36:27Z
dc.date.issued	2017
dc.description.abstract	Objective: A signature that unifies the colorectal cancer (CRC) microbiota across multiple studies has not been identified. In addition to methodological variance, heterogeneity may be caused by both microbial and host response differences, which was addressed in this study. Design: We prospectively studied the colonic microbiota and the expression of specific host response genes using faecal and mucosal samples (‘ON’ and ‘OFF’ the tumour, proximal and distal) from 59 patients undergoing surgery for CRC, 21 individuals with polyps and 56 healthy controls. Microbiota composition was determined by 16S rRNA amplicon sequencing; expression of host genes involved in CRC progression and immune response was quantified by real-time quantitative PCR. Results: The microbiota of patients with CRC differed from that of controls, but alterations were not restricted to the cancerous tissue. Differences between distal and proximal cancers were detected and faecal microbiota only partially reflected mucosal microbiota in CRC. Patients with CRC can be stratified based on higher level structures of mucosal-associated bacterial co-abundance groups (CAGs) that resemble the previously formulated concept of enterotypes. Of these, Bacteroidetes Cluster 1 and Firmicutes Cluster 1 were in decreased abundance in CRC mucosa, whereas Bacteroidetes Cluster 2, Firmicutes Cluster 2, Pathogen Cluster and Prevotella Cluster showed increased abundance in CRC mucosa. CRC-associated CAGs were differentially correlated with the expression of host immunoinflammatory response genes. Conclusions: CRC-associated microbiota profiles differ from those in healthy subjects and are linked with distinct mucosal gene-expression profiles. Compositional alterations in the microbiota are not restricted to cancerous tissue and differ between distal and proximal cancers.	en
dc.description.status	Peer reviewed	en
dc.description.version	Published Version	en
dc.format.mimetype	application/pdf	en
dc.identifier.citation	Flemer, B., Lynch, D. B., Brown, J. R. M., Jeffery, I. B., Ryan, F. J., Claesson, M. J., O'Riordain, M., Shanahan, F. and O'Toole, P. W. (2017) 'Tumour-associated and non-tumour-associated microbiota in colorectal cancer', Gut, 66(4), (12pp). doi: 10.1136/gutjnl-2015-309595	en
dc.identifier.doi	10.1136/gutjnl-2015-309595
dc.identifier.endpage	643
dc.identifier.issn	0017-5749
dc.identifier.issued	4
dc.identifier.journaltitle	Gut	en
dc.identifier.startpage	633
dc.identifier.uri	https://hdl.handle.net/10468/5383
dc.identifier.volume	66
dc.language.iso	en	en
dc.publisher	BMJ Publishing Group	en
dc.relation.project	info:eu-repo/grantAgreement/SFI/SFI Starting Investigator Research Grant (SIRG)/11/SIRG/B2162/IE/Molecular diagnostics and metatranscriptomics of colonic bacteria in inflammatory bowel disease/
dc.relation.project	info:eu-repo/grantAgreement/SFI/SFI Research Centres/12/RC/2273/IE/Alimentary Pharmabiotic Centre (APC) - Interfacing Food & Medicine/
dc.relation.uri	http://gut.bmj.com/content/66/4/633
dc.rights	© 2016, the Authors. Published by the BMJ Publishing Group Limited. For permission to use (where not already granted under a licence) please go to http://www.bmj.com/company/products-services/rights-and-licensing/. This is an Open Access article distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/	en
dc.rights.uri	https://creativecommons.org/licenses/by-nc/4.0/
dc.subject	Human gut microbiome	en
dc.subject	Colon-cancer	en
dc.subject	Tumorigenesis	en
dc.subject	Co-occurrence	en
dc.subject	Malignancy	en
dc.subject	Bacteria	en
dc.subject	Pcr	en
dc.title	Tumour-associated and non-tumour-associated microbiota in colorectal cancer	en
dc.type	Article (peer-reviewed)	en

Tumour-associated and non-tumour-associated microbiota in colorectal cancer

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