Evaluation of modulatory activities of Lactobacillus crispatus strains in the context of the vaginal microbiota

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dc.contributor.authorArgentini, C.en
dc.contributor.authorFontana, Federicoen
dc.contributor.authorAlessandri, Giuliaen
dc.contributor.authorLugli, Gabriele Andreaen
dc.contributor.authorMancabelli, Leonardoen
dc.contributor.authorOssiprandi, Maria Cristinaen
dc.contributor.authorvan Sinderen, Douween
dc.contributor.authorVentura, Marcoen
dc.contributor.authorMilani, Christianen
dc.contributor.authorTurroni, Francescaen
dc.contributor.funderGenProbio Srl
dc.contributor.funderScience Foundation Ireland
dc.contributor.funderUniversità degli Studi di Parma
dc.contributor.funderFondazione Cariparma
dc.date.accessioned2023-08-16T15:57:41Z
dc.date.available2023-08-16T16:03:30Zen
dc.date.available2023-08-16T15:57:41Z
dc.date.issued2022-04en
dc.date.updated2023-08-16T15:03:33Zen
dc.description.abstractIt has been widely reported that members of the genus Lactobacillus dominate the vaginal microbiota, which is represented by the most prevalent species Lactobacillus crispatus, Lactobacillus jensenii, Lactobacillus gasseri, and Lactobacillus iners. L. crispatus is furthermore considered an important microbial biomarker due to its professed beneficial implications on vaginal health. In order to identify molecular mechanisms responsible for health-promoting activities that are believed to be elicited by L. crispatus, we performed in silico investigations of the intraspecies biodiversity of vaginal microbiomes followed by in vitro experiments involving various L. crispatus strains along with other vaginal Lactobacillus species mentioned above. Specifically, we assessed their antibacterial activities against a variety of pathogenic microorganisms that are associated with vaginal infections. Moreover, coculture experiments of L. crispatus strains showing the most antibacterial activity against different pathogens revealed distinct ecological fitness and competitive properties with regard to other microbial colonizers. Interestingly, we observed that even phylogenetically closely related L. crispatus strains possess unique features in terms of their antimicrobial activities and associated competitive abilities, which suggests that they exert marked competition and evolutionary pressure within their specific environmental niche.IMPORTANCE The human vaginal microbiota includes all microorganisms that colonize the vaginal tract In this context, a vaginal microbiota dominated by Lactobacillus and specifically by Lactobacillus crispatus is considered a hallmark of health. The role of L. crispatus in maintaining host health is linked to its modulatory activity toward other members of the vaginal ecosystem and toward the host. In this study, in vitro experiments followed by genetic analyses of the mechanisms used by L. crispatus in colonizing the vaginal ecological niche, particularly in the production of different antimicrobial compounds, were evaluated, highlighting some intriguing novel aspects concerning the genetic variability of this species. Our results indicate that this species has adapted to its niche and may still undergo adaptation to enhance its competitiveness for niche colonization.en
dc.description.statusPeer revieweden
dc.description.versionPublished Version
dc.format.mimetypeapplication/pdfen
dc.identifier.citationArgentini, C., Fontana, F., Alessandri, G., Lugli, G.A., Mancabelli, L., Ossiprandi, M.C., Van Sinderen, D., Ventura, M., Milani, C. and Turroni, F. (2022) ‘Evaluation of modulatory activities of lactobacillus crispatus strains in the context of the vaginal microbiota’, Microbiology Spectrum, 10(2), (11pp). doi: 10.1128/spectrum.02733-21
dc.identifier.doi10.1128/spectrum.02733-21en
dc.identifier.eissn2165-0497
dc.identifier.endpage18
dc.identifier.issued2
dc.identifier.journaltitleMicrobiology Spectrum
dc.identifier.startpage1
dc.identifier.urihttps://hdl.handle.net/10468/14860
dc.identifier.volume10
dc.language.isoenen
dc.publisherAmerican Society for Microbiology
dc.relation.projectinfo:eu-repo/grantAgreement/SFI/SFI Research Centres/12/RC/2273/IE/Alimentary Pharmabiotic Centre (APC) - Interfacing Food & Medicine/
dc.rights© 2022 Argentini et al. This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjectClass IIA bacteriocins
dc.subjectLactic-acid bacteria
dc.subjectIn-vitro
dc.subjectAntibacterial activity
dc.subjectImmunity proteins
dc.subjectWomen
dc.subjectBifidobacteria
dc.subjectCommunities
dc.subjectConsistent
dc.subjectDiversity
dc.titleEvaluation of modulatory activities of Lactobacillus crispatus strains in the context of the vaginal microbiota
dc.typeArticle (peer-reviewed)
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