Genome analysis and characterisation of the exopolysaccharide produced by Bifidobacterium longum subsp. longum 35624™

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dc.contributor.authorAltmann, Friedrich
dc.contributor.authorKosma, Paul
dc.contributor.authorO'Callaghan, Amy
dc.contributor.authorLeahy, Sinead C.
dc.contributor.authorBottacini, Francesca
dc.contributor.authorMolloy, Evelyn M.
dc.contributor.authorPlattner, Stephan
dc.contributor.authorSchiavi, Elisa
dc.contributor.authorGleinser, Marita
dc.contributor.authorGroeger, David S.
dc.contributor.authorGrant, Ray
dc.contributor.authorRodriguez Perez, Noelia
dc.contributor.authorHealy, Selena
dc.contributor.authorSvehla, Elisabeth
dc.contributor.authorWindwarder, Markus
dc.contributor.authorHofinger, Andreas
dc.contributor.authorO'Connell Motherway, Mary
dc.contributor.authorAkdis, Cezmi A.
dc.contributor.authorXu, Jun
dc.contributor.authorRoper, Jennifer
dc.contributor.authorvan Sinderen, Douwe
dc.contributor.authorO'Mahony, Liam
dc.contributor.funderHealth Research Boarden
dc.contributor.funderIrish Research Council for Science, Engineering and Technologyen
dc.contributor.funderScience Foundation Irelanden
dc.contributor.funderEuropean Commissionen
dc.contributor.funderSchweizerischer Nationalfonds zur Förderung der Wissenschaftlichen Forschungen
dc.contributor.funderAlimentary Health Pharma Davos, Switzerlanden
dc.contributor.funderChristine Kühne Center for Allergy Research and Education, Switzerlanden
dc.contributor.funderProcter & Gamble, United States.en
dc.date.accessioned2016-10-06T11:55:44Z
dc.date.available2016-10-06T11:55:44Z
dc.date.issued2016-09-22
dc.description.abstractThe Bifibobacterium longum subsp. longum 35624™ strain (formerly named Bifidobacterium longum subsp. infantis) is a well described probiotic with clinical efficacy in Irritable Bowel Syndrome clinical trials and induces immunoregulatory effects in mice and in humans. This paper presents (a) the genome sequence of the organism allowing the assignment to its correct subspeciation longum; (b) a comparative genome assessment with other B. longum strains and (c) the molecular structure of the 35624 exopolysaccharide (EPS624). Comparative genome analysis of the 35624 strain with other B. longum strains determined that the sub-speciation of the strain is longum and revealed the presence of a 35624-specific gene cluster, predicted to encode the biosynthetic machinery for EPS624. Following isolation and acid treatment of the EPS, its chemical structure was determined using gas and liquid chromatography for sugar constituent and linkage analysis, electrospray and matrix assisted laser desorption ionization mass spectrometry for sequencing and NMR. The EPS consists of a branched hexasaccharide repeating unit containing two galactose and two glucose moieties, galacturonic acid and the unusual sugar 6-deoxy-L-talose. These data demonstrate that the B. longum 35624 strain has specific genetic features, one of which leads to the generation of a characteristic exopolysaccharide.en
dc.description.sponsorshipEuropean Commission (European Union Marie Curie training network, “TEAM-EPIC”); Swiss National Science Foundation (Grants project numbers CRSII3_154488 and 310030_144219); Christine Kühne Center for Allergy Research and Education; Science Foundation Ireland (SFI Grant No. SFI/12/RC/2273); Irish Research Council (Enterprise partnership scheme); Health Research Board (HRB postdoctoral fellowship (Grant No. PDTM/20011/9)); Alimentary Health Pharma Davos; Procter & Gamble, United States.en
dc.description.statusPeer revieweden
dc.description.versionPublished Versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.citationAltmann F., Kosma P., O’Callaghan A., Leahy S., Bottacini F. et al (2016) ‘Genome analysis and characterisation of the exopolysaccharide produced by Bifidobacterium longum subsp. longum 35624™,’ PLoS ONE 11(9): e0162983 (23pp). doi: 10.1371/journal.pone.0162983en
dc.identifier.doi10.1371/journal.pone.0162983
dc.identifier.endpagee0162983-23en
dc.identifier.issn1932-6203
dc.identifier.issued9en
dc.identifier.journaltitlePLoS ONEen
dc.identifier.startpagee0162983-1en
dc.identifier.urihttps://hdl.handle.net/10468/3164
dc.identifier.volume11en
dc.language.isoenen
dc.publisherPublic Library of Scienceen
dc.rights© 2016 Altmann et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.en
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/en
dc.subjectExopolysaccharidesen
dc.subjectBifidobacteriumen
dc.subjectComparative genomicsen
dc.subjectProtonsen
dc.subjectMonosaccharidesen
dc.subjectNMR spectroscopyen
dc.subjectGene predictionen
dc.subjectPolysaccharidesen
dc.titleGenome analysis and characterisation of the exopolysaccharide produced by Bifidobacterium longum subsp. longum 35624™en
dc.typeArticle (peer-reviewed)en
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