Genome analysis and characterisation of the exopolysaccharide produced by Bifidobacterium longum subsp. longum 35624™

Thumbnail Image
1314.PDF(3.66 MB)
Published Version
Altmann, Friedrich
Kosma, Paul
O'Callaghan, Amy
Leahy, Sinead C.
Bottacini, Francesca
Molloy, Evelyn M.
Plattner, Stephan
Schiavi, Elisa
Gleinser, Marita
Groeger, David S.
Journal Title
Journal ISSN
Volume Title
Public Library of Science
Research Projects
Organizational Units
Journal Issue
The Bifibobacterium longum subsp. longum 35624™ strain (formerly named Bifidobacterium longum subsp. infantis) is a well described probiotic with clinical efficacy in Irritable Bowel Syndrome clinical trials and induces immunoregulatory effects in mice and in humans. This paper presents (a) the genome sequence of the organism allowing the assignment to its correct subspeciation longum; (b) a comparative genome assessment with other B. longum strains and (c) the molecular structure of the 35624 exopolysaccharide (EPS624). Comparative genome analysis of the 35624 strain with other B. longum strains determined that the sub-speciation of the strain is longum and revealed the presence of a 35624-specific gene cluster, predicted to encode the biosynthetic machinery for EPS624. Following isolation and acid treatment of the EPS, its chemical structure was determined using gas and liquid chromatography for sugar constituent and linkage analysis, electrospray and matrix assisted laser desorption ionization mass spectrometry for sequencing and NMR. The EPS consists of a branched hexasaccharide repeating unit containing two galactose and two glucose moieties, galacturonic acid and the unusual sugar 6-deoxy-L-talose. These data demonstrate that the B. longum 35624 strain has specific genetic features, one of which leads to the generation of a characteristic exopolysaccharide.
Exopolysaccharides , Bifidobacterium , Comparative genomics , Protons , Monosaccharides , NMR spectroscopy , Gene prediction , Polysaccharides
Altmann F., Kosma P., O’Callaghan A., Leahy S., Bottacini F. et al (2016) ‘Genome analysis and characterisation of the exopolysaccharide produced by Bifidobacterium longum subsp. longum 35624™,’ PLoS ONE 11(9): e0162983 (23pp). doi: 10.1371/journal.pone.0162983
Link to publisher’s version