Behavioural and neurochemical consequences of chronic gut microbiota depletion during adulthood in the rat

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dc.contributor.authorHoban, Alan E.
dc.contributor.authorMoloney, Rachel D.
dc.contributor.authorGolubeva, Anna V.
dc.contributor.authorMcVey Neufeld, Karen A.
dc.contributor.authorO'Sullivan, Orla
dc.contributor.authorPatterson, Elaine
dc.contributor.authorStanton, Catherine
dc.contributor.authorDinan, Timothy G.
dc.contributor.authorClarke, Gerard
dc.contributor.authorCryan, John F.
dc.contributor.funderScience Foundation Irelanden
dc.contributor.funderHealth Research Boarden
dc.contributor.funderDepartment of Agriculture, Food and the Marineen
dc.date.accessioned2016-11-18T11:25:48Z
dc.date.available2016-11-18T11:25:48Z
dc.date.issued2016-10-11
dc.description.abstractGut microbiota colonization is a key event for host physiology that occurs early in life. Disruption of this process leads to altered brain development which ultimately manifests as changes in brain function and behaviour in adulthood. Studies using germ-free mice highlight the extreme impact on brain health that results from life without commensal microbes, however the impact of microbiota disturbances occurring in adulthood is less studied. To this end, we depleted the gut microbiota of 10-week-old male Sprague Dawley rats via chronic antibiotic treatment. Following this marked, sustained depletion of the gut bacteria, we investigated behavioural and molecular hallmarks of gut-brain communication. Our results reveal that depletion of the gut microbiota during adulthood results in deficits in spatial memory as tested by Morris water maze, increased visceral sensitivity and a greater display of depressive-like behaviours in the forced swim test. In tandem with these clear behavioural alterations we found change in altered CNS serotonin concentration along with changes in the mRNA levels of corticotrophin releasing hormone receptor 1 and glucocorticoid receptor. Additionally, we found changes in the expression of BDNF, a hallmark of altered microbiota-gut-brain axis signaling. In summary, this model of antibiotic-induced depletion of the gut microbiota can be used for future studies interested in the impact of the gut microbiota on host health without the confounding developmental influence of early-life microbial alterations.en
dc.description.statusPeer revieweden
dc.description.versionAccepted Versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.citationHoban, A. E., Moloney, R. D., Golubeva, A. V., McVey Neufeld, K. A., O’Sullivan, O., Patterson, E., Stanton, C., Dinan, T. G., Clarke, G. and Cryan, J. F. (2016) 'Behavioural and neurochemical consequences of chronic gut microbiota depletion during adulthood in the rat', Neuroscience, 339, pp. 463-477. doi: 10.1016/j.neuroscience.2016.10.003en
dc.identifier.doi10.1016/j.neuroscience.2016.10.003
dc.identifier.endpage477en
dc.identifier.issn0306-4522
dc.identifier.startpage463en
dc.identifier.urihttps://hdl.handle.net/10468/3290
dc.identifier.volume339en
dc.language.isoenen
dc.publisherElsevier Ltd.en
dc.relation.projectinfo:eu-repo/grantAgreement/SFI/SFI Research Centres/12/RC/2273/IE/Alimentary Pharmabiotic Centre (APC) - Interfacing Food & Medicine/
dc.relation.urihttp://www.sciencedirect.com/science/article/pii/S0306452217300143
dc.relation.urihttp://www.sciencedirect.com/science/article/pii/S0306452216305127
dc.rights© 2016, International Brain Research Organization. Published by Elsevier Ltd. All rights reserved. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/en
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/4.0/en
dc.subjectColonizationen
dc.subjectBrain developmenten
dc.subjectBrain functionen
dc.subjectBehaviouren
dc.subjectAntibioticen
dc.subjectDepletionen
dc.subjectGut bacteriaen
dc.subjectGut microbiotaen
dc.subjectGut-brain communicationen
dc.subjectSpatial memoryen
dc.subjectVisceral sensitivityen
dc.subjectSerotoninen
dc.subjectCognitionen
dc.subjectBrain monoaminesen
dc.subjectDepressive-like behavioursen
dc.titleBehavioural and neurochemical consequences of chronic gut microbiota depletion during adulthood in the raten
dc.typeArticle (peer-reviewed)en
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