The MpsB protein contributes to both the toxicity and immune evasion capacity of Staphylococcus aureus

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dc.contributor.authorDouglas, Edward J. A.
dc.contributor.authorDuggan, Seána
dc.contributor.authorBrignoli, Tarcisio
dc.contributor.authorMassey, Ruth C.
dc.contributor.funderUniversity of Bristolen
dc.contributor.funderBiotechnology and Biological Sciences Research Councilen
dc.contributor.funderWellcome Trusten
dc.date.accessioned2022-09-15T13:07:21Z
dc.date.available2022-09-15T13:07:21Z
dc.date.issued2021-10-07
dc.date.updated2022-09-15T12:05:57Z
dc.description.abstractUnderstanding the role specific bacterial factors play in the development of severe disease in humans is critical if new approaches to tackle such infections are to be developed. In this study we focus on genes we have found to be associated with patient outcome following bacteraemia caused by the major human pathogen Staphylococcus aureus. By examining the contribution these genes make to the ability of the bacteria to survive exposure to the antibacterial factors found in serum, we identify three novel serum resistance-associated genes, mdeA, mpsB and yycH. Detailed analysis of an MpsB mutant supports its previous association with the slow growing small colony variant (SCV) phenotype of S. aureus, and we demonstrate that the effect this mutation has on membrane potential prevents the activation of the Agr quorum sensing system, and as a consequence the mutant bacteria do not produce cytolytic toxins. Given the importance of both toxin production and immune evasion for the ability of S. aureus to cause disease, we believe that these findings explain the role of the mpsB gene as a mortality-associated locus during human disease.en
dc.description.sponsorshipUniversity of Bristol (PhD studentship); Wellcome Trust (grant number: 212258/Z/18/Z)en
dc.description.statusPeer revieweden
dc.description.versionPublished Versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.articleid001096en
dc.identifier.citationDouglas, E. J. A., Duggan, S., Brignoli, T. and Massey, R. C. (2021) 'The MpsB protein contributes to both the toxicity and immune evasion capacity of Staphylococcus aureus'. Microbiology, 167(10), 001096 (8pp). doi: 10.1099/mic.0.001096en
dc.identifier.doi10.1099/mic.0.001096en
dc.identifier.eissn1465-2080
dc.identifier.endpage8en
dc.identifier.issn1350-0872
dc.identifier.issued10en
dc.identifier.journaltitleAnnals of Microbiologyen
dc.identifier.startpage1en
dc.identifier.urihttps://hdl.handle.net/10468/13608
dc.identifier.volume167en
dc.language.isoenen
dc.publisherMicrobiology Societyen
dc.rights© 2021, the Authors. This is an open-access article distributed under the terms of the Creative Commons Attribution License. This article was made open access via a Publish and Read agreement between the Microbiology Society and the corresponding author’s institution.en
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/en
dc.subjectmpsBen
dc.subjectStaphylococcus aureusen
dc.subjectSerum survivalen
dc.subjectSmall colony variantsen
dc.titleThe MpsB protein contributes to both the toxicity and immune evasion capacity of Staphylococcus aureusen
dc.typeArticle (peer-reviewed)en
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