Transcriptome profiling defines a novel regulon modulated by the LysR-type transcriptional regulator MexT in Pseudomonas aeruginosa

Tian, Zhe-Xian; Fargier, Emilie; Mac Aogáin, Micheál; Adams, Claire; Wang, Yi-Ping; O'Gara, Fergal

Transcriptome profiling defines a novel regulon modulated by the LysR-type transcriptional regulator MexT in Pseudomonas aeruginosa

dc.contributor.author	Tian, Zhe-Xian
dc.contributor.author	Fargier, Emilie
dc.contributor.author	Mac Aogáin, Micheál
dc.contributor.author	Adams, Claire
dc.contributor.author	Wang, Yi-Ping
dc.contributor.author	O'Gara, Fergal
dc.contributor.funder	European Commission
dc.contributor.funder	Science Foundation Ireland
dc.contributor.funder	Department of Agriculture, Food and the Marine
dc.contributor.funder	Irish Research Council for Science, Engineering and Technology
dc.contributor.funder	Health Research Board
dc.contributor.funder	Environmental Protection Agency
dc.contributor.funder	National Natural Science Foundation of China
dc.date.accessioned	2017-11-14T13:24:32Z
dc.date.available	2017-11-14T13:24:32Z
dc.date.issued	2009
dc.description.abstract	The LysR-family regulator MexT modulates the expression of the MexEF-OprN efflux system in the human pathogen Pseudomonas aeruginosa. Recently, we demonstrated that MexT regulates certain virulence phenotypes, including the type-three secretion system and early attachment independent of its role in regulating MexEF-OprN. In this study, transcriptome profiling was utilized to investigate the global nature of MexT regulation in P. aeruginosa PAO1 and an isogenic mexEF mutant. Twelve genes of unknown function were highly induced by overexpressing MexT independent of MexEF-OprN. A well-conserved DNA motif was identified in the upstream regulatory region of nine of these genes and upstream of mexE. Reporter fusion analysis demonstrated that the expression of the genes was significantly induced by MexT in P. aeruginosa and a heterogenous Escherichia coli strain and that the conserved sequence was required for this induction. The conserved DNA motif was further characterized as the MexT binding site by site-directed mutagenesis and electrophoretic mobility shift assays. Genes containing this conserved regulatory sequence were identified across other Pseudomonas species, and their expression was activated by MexT. Thus, a novel regulon directly modulated by MexT, that includes but is independent of mexEF-oprN, has been identified.	en
dc.description.sponsorship	European Commission (MTKD-CT-2006-042062; O36314); Department of Agriculture, Food and the Marine (DAF RSF 06 321; DAF RSF 06 377); Irish Research Council for Science; Engineering and Technology (05/EDIV/FP107); Health Research Board (RP/2006/271; RP/2007/290); Environmental Protection Agency (2006-PhD-S-21; 2008-PhD-S-2); National Natural Science Foundation of China (30830005)	en
dc.description.status	Peer reviewed	en
dc.description.version	Published Version	en
dc.format.mimetype	application/pdf	en
dc.identifier.citation	Tian, Z.-X., Fargier, E., Mac Aogáin, M., Adams, C., Wang, Y.-P. and O’Gara, F. (2009) 'Transcriptome profiling defines a novel regulon modulated by the LysR-type transcriptional regulator MexT in Pseudomonas aeruginosa', Nucleic Acids Research, 37(22), pp. 7546-7559. doi: 10.1093/nar/gkp828	en
dc.identifier.doi	10.1093/nar/gkp828
dc.identifier.endpage	7559
dc.identifier.issn	0305-1048
dc.identifier.issued	22
dc.identifier.journaltitle	Nucleic Acids Research	en
dc.identifier.startpage	7546
dc.identifier.uri	https://hdl.handle.net/10468/5030
dc.identifier.volume	37
dc.language.iso	en	en
dc.publisher	Oxford University Press	en
dc.relation.project	info:eu-repo/grantAgreement/SFI/SFI Research Frontiers Programme (RFP)/08/RFP/GEN1295/IE/Functional Genomics of a novel regulon co-ordinating virulence phenotypes in Pseudomonas aeruginosa - Host interactions/
dc.relation.project	info:eu-repo/grantAgreement/SFI/SFI Principal Investigator Programme (PI)/07/IN.1/B948/IE/A Systems approach to elucidate the mechanisms of RsmA mediated regulation of virulence-associated traits in Pseudomonas aeruginosa in response to environmental and host interactions/
dc.relation.uri	https://academic.oup.com/nar/article-lookup/doi/10.1093/nar/gkp828
dc.rights	© 2009, the Authors. Published by Oxford University Press. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc/2.5/uk/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.	en
dc.rights.uri	http://creativecommons.org/licenses/by-nc/2.5/uk/
dc.subject	Multidrug efflux system	en
dc.subject	Differential selection	en
dc.subject	Xenobiotic reductases	en
dc.subject	OprN	en
dc.subject	Resistance	en
dc.subject	Expression	en
dc.subject	Proteins	en
dc.subject	Pumps	en
dc.subject	Overexpression	en
dc.subject	Identification	en
dc.title	Transcriptome profiling defines a novel regulon modulated by the LysR-type transcriptional regulator MexT in Pseudomonas aeruginosa	en
dc.type	Article (peer-reviewed)	en