Devil's Claw to suppress appetite - ghrelin receptor modulation potential of a Harpagophytum procumbens root extract

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dc.contributor.authorTorres-Fuentes, Cristina
dc.contributor.authorTheeuwes, Wessel F.
dc.contributor.authorMcMullen, Michael K.
dc.contributor.authorMcMullen, Anna K.
dc.contributor.authorDinan, Timothy G.
dc.contributor.authorCryan, John F.
dc.contributor.authorSchellekens, Harriƫt
dc.contributor.funderEnterprise Ireland
dc.contributor.funderScience Foundation Ireland
dc.date.accessioned2016-02-17T11:43:39Z
dc.date.available2016-02-17T11:43:39Z
dc.date.issued2014
dc.description.abstractGhrelin is a stomach-derived peptide that has been identified as the only circulating hunger hormone that exerts a potent orexigenic effect via activation of its receptor, the growth hormone secretagogue receptor (GHS-R1a). Hence, the ghrelinergic system represents a promising target to treat obesity and obesity-related diseases. In this study we analysed the GHS-R1a receptor activating potential of Harpagophytum procumbens, popularly known as Devil's Claw, and its effect on food intake in vivo. H. procumbens is an important traditional medicinal plant from Southern Africa with potent anti-inflammatory and analgesic effects. This plant has been also used as an appetite modulator but most evidences are anecdotal and to our knowledge, no clear scientific studies relating to appetite modulation have been done to this date. The ghrelin receptor activation potential of an extract derived from the dried tuberous roots of H. procumbens was analysed by calcium mobilization and receptor internalization assays in human embryonic kidney cells (Hek) stably expressing the GHS-R1a receptor. Food intake was investigated in male C57BL/6 mice following intraperitoneal administration of H. procumbens root extract in ad libitum and food restricted conditions. Exposure to H. procumbens extract demonstrated a significant increased cellular calcium influx but did not induce subsequent GHS-R1a receptor internalization, which is a characteristic for full receptor activation. A significant anorexigenic effect was observed in male C57BL/6 mice following peripheral administration of H. procumbens extract. We conclude that H. procumbens root extract is a potential novel source for potent anti-obesity bioactives. These results reinforce the promising potential of natural bioactives to be developed into functional foods with weight-loss and weight maintenance benefits.en
dc.description.sponsorshipEnterprise Ireland (Grant No. CC20080001); Science Foundation Ireland (SFI Grant No. 02/CE/B124; 07/CE/B1368; Centre Grant to Alimentary Pharmabiotic Centre, University College Cork)en
dc.description.statusPeer revieweden
dc.description.versionPublished Versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.articleide103118
dc.identifier.citationTorres-Fuentes C, Theeuwes WF, McMullen MK, McMullen AK, Dinan TG, Cryan JF, et al. (2014) Devil's Claw to Suppress Appetiteā€”Ghrelin Receptor Modulation Potential of a Harpagophytum procumbens Root Extract. PLoS ONE 9(7): e103118. doi:10.1371/journal.pone.0103118
dc.identifier.doi10.1371/journal.pone.0103118
dc.identifier.issn1932-6203
dc.identifier.issued7en
dc.identifier.journaltitlePLOS ONEen
dc.identifier.urihttps://hdl.handle.net/10468/2329
dc.identifier.volume9en
dc.language.isoenen
dc.publisherPublic Library of Scienceen
dc.rightsĀ© 2015 Torres-Fuentes et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are crediteden
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.subjectDietary fiberen
dc.subjectHormoneen
dc.subjectDesensitizationen
dc.subjectHarpagosideen
dc.subjectArrestinsen
dc.subjectPeptidesen
dc.subjectProteinsen
dc.subjectStomachen
dc.subjectAgonisten
dc.subjectObesityen
dc.titleDevil's Claw to suppress appetite - ghrelin receptor modulation potential of a Harpagophytum procumbens root extracten
dc.typeArticle (peer-reviewed)en
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