Bifidobacterium breve UCC2003 exopolysaccharide modulates the early life Microbiota by acting as a potential dietary substrate

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dc.contributor.authorPüngel, Deborah
dc.contributor.authorTreveil, Agatha
dc.contributor.authorDalby, Matthew J.
dc.contributor.authorCaim, Shabhonam
dc.contributor.authorColquhoun, Ian J.
dc.contributor.authorBooth, Catherine
dc.contributor.authorKetskemety, Jennifer
dc.contributor.authorKorcsmaros, Tamas
dc.contributor.authorvan Sinderen, Douwe
dc.contributor.authorLawson, Melissa A. E.
dc.contributor.authorHall, Lindsay J.
dc.contributor.funderWellcome Trust
dc.contributor.funderH2020 Marie Skłodowska-Curie Actions
dc.contributor.funderScience Foundation Ireland
dc.date.accessioned2023-09-04T08:35:08Z
dc.date.available2023-09-01T15:45:02Zen
dc.date.available2023-09-04T08:35:08Z
dc.date.issued2020
dc.date.updated2023-09-01T14:45:05Zen
dc.description.abstractBACKGROUND: Bifidobacterium represents an important early life microbiota member. Specific bifidobacterial components, exopolysaccharides (EPS), positively modulate host responses, with purified EPS also suggested to impact microbe-microbe interactions by acting as a nutrient substrate. Thus, we determined the longitudinal effects of bifidobacterial EPS on microbial communities and metabolite profiles using an infant model colon system. METHODS: Differential gene expression and growth characteristics were determined for each strain; Bifidobacterium breve UCC2003 and corresponding isogenic EPS-deletion mutant (B. breve UCC2003del). Model colon vessels were inoculated with B. breve and microbiome dynamics monitored using 16S rRNA sequencing and metabolomics (NMR). RESULTS: Transcriptomics of EPS mutant vs. B. breve UCC2003 highlighted discrete differential gene expression (e.g., eps biosynthetic cluster), though overall growth dynamics between strains were unaffected. The EPS-positive vessel had significant shifts in microbiome and metabolite profiles until study end (405 h); with increases of Tyzzerella and Faecalibacterium, and short-chain fatty acids, with further correlations between taxa and metabolites which were not observed within the EPS-negative vessel. CONCLUSIONS: These data indicate that B. breve UCC2003 EPS is potentially metabolized by infant microbiota members, leading to differential microbial metabolism and altered metabolite by-products. Overall, these findings may allow development of EPS-specific strategies to promote infant health.en
dc.description.statusPeer revieweden
dc.description.versionPublished Version
dc.format.mimetypeapplication/pdfen
dc.identifier.articleid948
dc.identifier.citationPüngel, D., Treveil, A., Dalby, M. J., Caim, S., Colquhoun, I. J., Booth, C., Ketskemety, J., Korcsmaros, T., Van Sinderen, D., Lawson, M. A., & Hall, L. J. (2020). 'Bifidobacterium breve ucc2003 exopolysaccharide modulates the early life microbiota by acting as a potential dietary substrate', Nutrients, 12(4), 948 (17pp). doi: 10.3390/nu12040948
dc.identifier.doi10.3390/nu12040948en
dc.identifier.eissn2072-6643
dc.identifier.endpage17
dc.identifier.issued4
dc.identifier.journaltitleNutrients
dc.identifier.startpage1
dc.identifier.urihttps://hdl.handle.net/10468/14901
dc.identifier.volume12
dc.language.isoenen
dc.publisherMDPI
dc.relation.projectinfo:eu-repo/grantAgreement/EC/H2020::MSCA-IF-EF-ST/661594/EU/Elucidating how Bifidobacteria shapes the microbiota in response to infant diet./INFANT MICROBIOTA
dc.relation.projectWellcome Trust (Investigator award (100974/C/13/Z))
dc.relation.projectinfo:eu-repo/grantAgreement/SFI/SFI Research Centres/12/RC/2273/IE/Alimentary Pharmabiotic Centre (APC) - Interfacing Food & Medicine/
dc.rights© 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subject16S rRNA profiling
dc.subjectBifidobacterium
dc.subjectCross-feeding
dc.subjectDiet
dc.subjectEarly life
dc.subjectExopolysaccharides
dc.subjectMetabolomics
dc.subjectModel colon
dc.titleBifidobacterium breve UCC2003 exopolysaccharide modulates the early life Microbiota by acting as a potential dietary substrate
dc.typeArticle (peer-reviewed)en
dc.typeArticleen
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