Bifidobacterium breve UCC2003 exopolysaccharide modulates the early life Microbiota by acting as a potential dietary substrate

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Püngel, Deborah
Treveil, Agatha
Dalby, Matthew J.
Caim, Shabhonam
Colquhoun, Ian J.
Booth, Catherine
Ketskemety, Jennifer
Korcsmaros, Tamas
van Sinderen, Douwe
Lawson, Melissa A. E.
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BACKGROUND: Bifidobacterium represents an important early life microbiota member. Specific bifidobacterial components, exopolysaccharides (EPS), positively modulate host responses, with purified EPS also suggested to impact microbe-microbe interactions by acting as a nutrient substrate. Thus, we determined the longitudinal effects of bifidobacterial EPS on microbial communities and metabolite profiles using an infant model colon system. METHODS: Differential gene expression and growth characteristics were determined for each strain; Bifidobacterium breve UCC2003 and corresponding isogenic EPS-deletion mutant (B. breve UCC2003del). Model colon vessels were inoculated with B. breve and microbiome dynamics monitored using 16S rRNA sequencing and metabolomics (NMR). RESULTS: Transcriptomics of EPS mutant vs. B. breve UCC2003 highlighted discrete differential gene expression (e.g., eps biosynthetic cluster), though overall growth dynamics between strains were unaffected. The EPS-positive vessel had significant shifts in microbiome and metabolite profiles until study end (405 h); with increases of Tyzzerella and Faecalibacterium, and short-chain fatty acids, with further correlations between taxa and metabolites which were not observed within the EPS-negative vessel. CONCLUSIONS: These data indicate that B. breve UCC2003 EPS is potentially metabolized by infant microbiota members, leading to differential microbial metabolism and altered metabolite by-products. Overall, these findings may allow development of EPS-specific strategies to promote infant health.
16S rRNA profiling , Bifidobacterium , Cross-feeding , Diet , Early life , Exopolysaccharides , Metabolomics , Model colon
Püngel, D., Treveil, A., Dalby, M. J., Caim, S., Colquhoun, I. J., Booth, C., Ketskemety, J., Korcsmaros, T., Van Sinderen, D., Lawson, M. A., & Hall, L. J. (2020). 'Bifidobacterium breve ucc2003 exopolysaccharide modulates the early life microbiota by acting as a potential dietary substrate', Nutrients, 12(4), 948 (17pp). doi: 10.3390/nu12040948