Drunk bugs: chronic vapour alcohol exposure induces marked changes in the gut microbiome in mice

dc.contributor.authorPeterson, Veronica L.
dc.contributor.authorJury, Nicholas J.
dc.contributor.authorCabrera-Rubio, Raul
dc.contributor.authorDraper, Lorraine A.
dc.contributor.authorCrispie, Fiona
dc.contributor.authorCotter, Paul D.
dc.contributor.authorDinan, Timothy G.
dc.contributor.authorHolmes, Andrew
dc.contributor.authorCryan, John F.
dc.contributor.funderScience Foundation Irelanden
dc.contributor.funderNational Institute on Alcohol Abuse and Alcoholismen
dc.date.accessioned2017-08-17T11:37:45Z
dc.date.available2017-08-17T11:37:45Z
dc.date.issued2017-02-01
dc.date.updated2017-08-17T11:20:10Z
dc.description.abstractThe gut microbiota includes a community of bacteria that play an integral part in host health and biological processes. Pronounced and repeated findings have linked gut microbiome to stress, anxiety, and depression. Currently, however, there remains only a limited set of studies focusing on microbiota change in substance abuse, including alcohol use disorder. To date, no studies have investigated the impact of vapour alcohol administration on the gut microbiome. For research on gut microbiota and addiction to proceed, an understanding of how route of drug administration affects gut microbiota must first be established. Animal models of alcohol abuse have proven valuable for elucidating the biological processes involved in addiction and alcohol-related diseases. This is the first study to investigate the effect of vapour route of ethanol administration on gut microbiota in mice. Adult male C57BL/6J mice were exposed to 4 weeks of chronic intermittent vapourized ethanol (CIE, N = 10) or air (Control, N = 9). Faecal samples were collected at the end of exposure followed by 16S sequencing and bioinformatic analysis. Robust separation between CIE and Control was seen in the microbiome, as assessed by alpha (p < 0.05) and beta (p < 0.001) diversity, with a notable decrease in alpha diversity in CIE. These results demonstrate that CIE exposure markedly alters the gut microbiota in mice. Significant increases in genus Alistipes (p < 0.001) and significant reductions in genra Clostridium IV and XIVb (p < 0.001), Dorea (p < 0.01), and Coprococcus (p < 0.01) were seen between CIE mice and Control. These findings support the viability of the CIE method for studies investigating the microbiota-gut-brain axis and align with previous research showing similar microbiota alterations in inflammatory states during alcoholic hepatitis and psychological stress.en
dc.description.sponsorshipScience Foundation Ireland (Grant No. 12/RC/2273); National Institute on Alcohol Abuse and Alcoholism (Intramural Research Program)en
dc.description.statusPeer revieweden
dc.description.versionAccepted Versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.citationPeterson, V. L., Jury, N. J., Cabrera-Rubio, R., Draper, L. A., Crispie, F., Cotter, P. D., Dinan, T. G., Holmes, A. and Cryan, J. F. (2017) 'Drunk bugs: chronic vapour alcohol exposure induces marked changes in the gut microbiome in mice', Behavioural Brain Research, 323. pp. 172-176. doi:10.1016/j.bbr.2017.01.049en
dc.identifier.doi10.1016/j.bbr.2017.01.049
dc.identifier.endpage176en
dc.identifier.issn0166-4328
dc.identifier.journaltitleBehavioural Brain Researchen
dc.identifier.startpage172en
dc.identifier.urihttps://hdl.handle.net/10468/4465
dc.identifier.volume323en
dc.language.isoenen
dc.publisherElsevier Ltden
dc.rights© 2017, Elsevier B.V. All rights reserved. This manuscript version is made available under the CC-BY-NC-ND 4.0 licenseen
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0en
dc.subjectChronic alcoholen
dc.subjectMicrobiomeen
dc.subjectVapour ethanolen
dc.subjectMicrobiota-gut-brain axisen
dc.titleDrunk bugs: chronic vapour alcohol exposure induces marked changes in the gut microbiome in miceen
dc.typeArticle (peer-reviewed)en
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