A yeast-based biosensor for screening of short- and medium-chain fatty acid production

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dc.contributor.authorBaumann, Leonie
dc.contributor.authorRajkumar, Arun S.
dc.contributor.authorMorrissey, John P.
dc.contributor.authorBoles, Eckhard
dc.contributor.authorOreb, Mislav
dc.contributor.funderHorizon 2020en
dc.date.accessioned2019-09-02T14:36:33Z
dc.date.available2019-09-02T14:36:33Z
dc.date.issued2018-10-19
dc.description.abstractShort- and medium-chain fatty acids (SMCFA) are important platform chemicals currently produced from nonsustainable resources. The engineering of microbial cells to produce SMCFA, however, lacks high-throughput methods to screen for best performing cells. Here, we present the development of a whole-cell biosensor for easy and rapid detection of SMCFA. The biosensor is based on a multicopy yeast plasmid containing the SMCFA-responsive PDR12 promoter coupled to GFP as the reporter gene. The sensor detected hexanoic, heptanoic and octanoic acid over a linear range up to 2, 1.5, and 0.75 mM, respectively, but did not show a linear response to decanoic and dodecanoic acid. We validated the functionality of the biosensor with culture supernatants of a previously engineered Saccharomyces cerevisiae octanoic acid producer strain and derivatives thereof. The biosensor signal correlated strongly with the octanoic acid concentrations as determined by gas chromatography. Thus, this biosensor enables the high-throughput screening of SMCFA producers and has the potential to drastically speed up the engineering of diverse SMCFA producing cell factories.en
dc.description.statusPeer revieweden
dc.description.versionPublished Versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.citationBaumann, L., Rajkumar, A.S., Morrissey, J.P., Boles, E. and Oreb, M., 2018. A Yeast-Based Biosensor for Screening of Short-and Medium-Chain Fatty Acid Production. ACS synthetic biology, 7(11), (7pp). DOI:10.1021/acssynbio.8b00309en
dc.identifier.doi10.1021/acssynbio.8b00309en
dc.identifier.eissn2161-5063
dc.identifier.endpage2646en
dc.identifier.issn2161-5063
dc.identifier.issued11en
dc.identifier.journaltitleACS Synthetic Biologyen
dc.identifier.startpage2640en
dc.identifier.urihttps://hdl.handle.net/10468/8434
dc.identifier.volume7en
dc.language.isoenen
dc.publisherAmerican Chemical Societyen
dc.relation.projectinfo:eu-repo/grantAgreement/EC/H2020::RIA/720824/EU/Model-Based Construction And Optimisation Of Versatile Chassis Yeast Strains For Production Of Valuable Lipid And Aromatic Compounds/CHASSYen
dc.relation.urihttps://pubs.acs.org/doi/10.1021/acssynbio.8b00309
dc.rights© 2018 American Chemical Societyen
dc.rights.urihttp://www.copyright.com/en
dc.subjectBiosensoren
dc.subjectHigh-throughput screeningen
dc.subjectShort-chain fatty acidsen
dc.subjectMedium-chain fatty acidsen
dc.subjectOctanoic aciden
dc.subjectPDR12en
dc.titleA yeast-based biosensor for screening of short- and medium-chain fatty acid productionen
dc.typeArticle (peer-reviewed)en
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