Phenotypic and genetic analyses of two Campylobacter fetus isolates from a patient with relapsed prosthetic valve endocarditis.

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dc.contributor.authorLynch, Caoimhe T.
dc.contributor.authorButtimer, Colin
dc.contributor.authorEpping, L.ennard
dc.contributor.authorO'Connor, James
dc.contributor.authorWalsh, Niamh
dc.contributor.authorMcCarthy, Conor
dc.contributor.authorO'Brien, Deirdre
dc.contributor.authorVaughan, Carl
dc.contributor.authorSemmler, Torsten
dc.contributor.authorBolton, Declan
dc.contributor.authorCoffey, Aidan
dc.contributor.authorLucey, Brigid
dc.contributor.funderDepartment of Agriculture, Food and the Marine, Irelanden
dc.contributor.funderFood Institutional Research Measureen
dc.contributor.funderBiotechnology and Biological Sciences Research Councilen
dc.contributor.funderTeagascen
dc.date.accessioned2022-02-22T16:33:25Z
dc.date.available2022-02-22T16:33:25Z
dc.date.issued2021-12-28
dc.date.updated2022-02-22T16:18:58Z
dc.description.abstractCampylobacter fetus can cause intestinal and systemic disease in humans and are well-established veterinary and economic pathogens. We report the complete genomic sequences of two C. fetus subsp. fetus (Cff) isolates recovered in 2017 (CITCf01) and 2018 (CITCf02) from a case of recurrent prosthetic valve endocarditis. Both were capable of growth aerobically. Their genomes were found to be highly conserved and syntenic with 99.97% average nucleotide identity (ANI) while differences in their respective sap loci defined the temporal separation of their genomes. Based on core genome phylogeny and ANI of 83 Cff genomes belonging to the previously described human-associated Cff lineage, CITCf01 and CITCf02 grouped in a clade of 11 sequence type (ST)3 Cff (including the Cff type strain NCTC 10842T). CITCf01 and CITCf02 were marked for their lack of unique genomic features when compared to isolates within the subspecies and the type strain in particular. We identified point mutations in oxidative stress response genes, among others, that may contribute to aerobiosis. We report a case of Cff causing relapsed prosthetic valve endocarditis and we highlight the sap island as a polymorphic site within the genetically stable ST3 lineage, central to pathogenicity.en
dc.description.sponsorshipDepartment of Agriculture Food and the Marine, Ireland (Food Institutional Research Measure (grant number 15F641)); Teagasc (Teagasc Walsh Scholarship (grant number 2017265)); Biotechnology and Biological Sciences Research Council (BBSRC genome sequencing was provided by MicrobesNG (http://www.microbesng.uk) supported by the BBSRC (grant number BB/L024209/1))en
dc.description.statusPeer revieweden
dc.description.versionAccepted Versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.citationLynch, C. T., Buttimer, C., Epping, L., O'Connor, J., Walsh, N., Mccarthy, C., O'Brien, D., Vaughan, C., Semmler, T., Bolton, D., Coffey, A., Lucey, B. (2022) ‘Phenotypic and genetic analyses of two Campylobacter fetus isolates from a patient with relapsed prosthetic valve endocarditis’, Pathogens and Disease, 79 (9), pp. 1-12. doi:10.1093/femspd/ftab055en
dc.identifier.doi10.1093/femspd/ftab055en
dc.identifier.endpage12en
dc.identifier.issn2049-632X
dc.identifier.issued9en
dc.identifier.journaltitlePathogens And Diseaseen
dc.identifier.startpage1en
dc.identifier.urihttps://hdl.handle.net/10468/12590
dc.identifier.volume79en
dc.language.isoenen
dc.publisherOxford University Pressen
dc.rights© The Author(s) 2021. Published by Oxford University Press on behalf of FEMS. This is a pre-copyedited, author-produced version of an article accepted for publication in Pathogens and Disease following peer review. The version of record is available online at: https://doi.org/10.1093/femspd/ftab055en
dc.subjectPhylogenomicsen
dc.subjectEndocarditisen
dc.subjectCampylobacter fetusen
dc.subjectCampylobacteren
dc.subjectAerobiosisen
dc.subjectPhenotypeen
dc.titlePhenotypic and genetic analyses of two Campylobacter fetus isolates from a patient with relapsed prosthetic valve endocarditis.en
dc.typeArticle (peer-reviewed)en
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