In vitro and in vivo assessment of the potential of Escherichia coli phages to treat infections and survive gastric conditions
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Published Version
Date
2021
Authors
Kaczorowska, Joanna
Casey, Eoghan
Lugli, Gabriele A.
Ventura, Marco
Clarke, David J.
van Sinderen, Douwe
Mahony, Jennifer
Journal Title
Journal ISSN
Volume Title
Publisher
MDPI
Published Version
Abstract
Enterotoxigenic Escherichia coli (ETEC) and Shigella ssp. infections are associated with high rates of mortality, especially in infants in developing countries. Due to increasing levels of global antibiotic resistance exhibited by many pathogenic organisms, alternative strategies to combat such infections are urgently required. In this study, we evaluated the stability of five coliphages (four Myoviridae and one Siphoviridae phage) over a range of pH conditions and in simulated gastric conditions. The Myoviridae phages were stable across the range of pH 2 to 7, while the Siphoviridae phage, JK16, exhibited higher sensitivity to low pH. A composite mixture of these five phages was tested in vivo in a Galleria mellonella model. The obtained data clearly shows potential in treating E. coli infections prophylactically.
Description
Keywords
Phage therapy , Prophylactic , Infections , Galleria mellonella , Diarrheagenic Escherichia coli
Citation
Kaczorowska, J., Casey, E., Lugli, G.A., Ventura, M., Clarke, D.J., Van Sinderen, D. and Mahony, J. (2021) ‘In vitro and in vivo assessment of the potential of escherichia coli phages to treat infections and survive gastric conditions’, Microorganisms, 9(9), 1869 (15pp). doi: 10.3390/microorganisms9091869