In vitro and in vivo assessment of the potential of Escherichia coli phages to treat infections and survive gastric conditions

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dc.contributor.authorKaczorowska, Joannaen
dc.contributor.authorCasey, Eoghanen
dc.contributor.authorLugli, Gabriele A.en
dc.contributor.authorVentura, Marcoen
dc.contributor.authorClarke, David J.en
dc.contributor.authorvan Sinderen, Douween
dc.contributor.authorMahony, Jenniferen
dc.contributor.funderBill and Melinda Gates Foundationen
dc.contributor.funderScience Foundation Irelanden
dc.date.accessioned2023-11-16T14:17:26Z
dc.date.available2023-11-16T14:17:26Z
dc.date.issued2021en
dc.description.abstractEnterotoxigenic Escherichia coli (ETEC) and Shigella ssp. infections are associated with high rates of mortality, especially in infants in developing countries. Due to increasing levels of global antibiotic resistance exhibited by many pathogenic organisms, alternative strategies to combat such infections are urgently required. In this study, we evaluated the stability of five coliphages (four Myoviridae and one Siphoviridae phage) over a range of pH conditions and in simulated gastric conditions. The Myoviridae phages were stable across the range of pH 2 to 7, while the Siphoviridae phage, JK16, exhibited higher sensitivity to low pH. A composite mixture of these five phages was tested in vivo in a Galleria mellonella model. The obtained data clearly shows potential in treating E. coli infections prophylactically.en
dc.description.sponsorshipBill and Melinda Gates Foundation (OPP1150567)en
dc.description.statusPeer revieweden
dc.description.versionPublished Versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.articleid1869en
dc.identifier.citationKaczorowska, J., Casey, E., Lugli, G.A., Ventura, M., Clarke, D.J., Van Sinderen, D. and Mahony, J. (2021) ‘In vitro and in vivo assessment of the potential of escherichia coli phages to treat infections and survive gastric conditions’, Microorganisms, 9(9), 1869 (15pp). doi: 10.3390/microorganisms9091869en
dc.identifier.doi10.3390/microorganisms9091869en
dc.identifier.endpage15en
dc.identifier.issn2076-2607en
dc.identifier.issued9en
dc.identifier.journaltitleMicroorganismsen
dc.identifier.startpage1en
dc.identifier.urihttps://hdl.handle.net/10468/15238
dc.identifier.volume9en
dc.language.isoenen
dc.publisherMDPIen
dc.relation.ispartofMicroorganismsen
dc.relation.projectinfo:eu-repo/grantAgreement/SFI/SFI Starting Investigator Research Grant (SIRG)/15/SIRG/3430/IE/Phage-host interactome of the dairy bacterium Streptococcus thermophilus (PHIST)/en
dc.relation.projectinfo:eu-repo/grantAgreement/SFI/SFI Investigator Programme/13/IA/1953/IE/Functional analysis of the host adsorption and DNA injection processes of a lactococcal bacteriophage/en
dc.rights© 2021, by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https:// creativecommons.org/licenses/by/ 4.0/)en
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/en
dc.subjectPhage therapyen
dc.subjectProphylacticen
dc.subjectInfectionsen
dc.subjectGalleria mellonellaen
dc.subjectDiarrheagenic Escherichia colien
dc.titleIn vitro and in vivo assessment of the potential of Escherichia coli phages to treat infections and survive gastric conditionsen
dc.typeArticle (peer-reviewed)en
dc.typejournal-articleen
oaire.citation.issue9en
oaire.citation.volume9en
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