Unveiling the gut microbiota composition and functionality associated with constipation through metagenomic analyses

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dc.contributor.authorMancabelli, Leonardo
dc.contributor.authorMilani, Christian
dc.contributor.authorLugli, Gabriele A.
dc.contributor.authorTurroni, Francesca
dc.contributor.authorMangifesta, Marta
dc.contributor.authorViappiani, Alice
dc.contributor.authorTicinesi, Andrea
dc.contributor.authorNouvenne, Antonio
dc.contributor.authorMeschi, Tiziana
dc.contributor.authorvan Sinderen, Douwe
dc.contributor.authorVentura, Marco
dc.contributor.funderHorizon 2020 Framework Programme
dc.contributor.funderUniversità degli Studi di Parma
dc.contributor.funderFondazione Cariparma
dc.contributor.funderScience Foundation Ireland
dc.date.accessioned2017-09-26T11:39:24Z
dc.date.available2017-09-26T11:39:24Z
dc.date.issued2017
dc.description.abstractFunctional constipation (FC) is a gastrointestinal disorder with a high prevalence among the general population. The precise causes of FC are still unknown and are most likely multifactorial. Growing evidence indicates that alterations of gut microbiota composition contribute to constipation symptoms. Nevertheless, many discrepancies exist in literature and no clear link between FC and gut microbiota composition has as yet been identified. In this study, we performed 16 S rRNA-based microbial profiling analysis of 147 stool samples from 68 FC individuals and compared their microbial profiles with those of 79 healthy subjects (HS). Notably, the gut microbiota of FC individuals was shown to be depleted of members belonging to Bacteroides, Roseburia and Coprococcus 3. Furthermore, the metabolic capabilities of the gut microbiomes of five FC and five HS individuals were evaluated through shotgun metagenomics using a MiSeq platform, indicating that HS are enriched in pathways involved in carbohydrate, fatty acid and lipid metabolism as compared to FC. In contrast, the microbiomes corresponding to FC were shown to exhibit high abundance of genes involved in hydrogen production, methanogenesis and glycerol degradation. The identified differences in bacterial composition and metabolic capabilities may play an important role in development of FC symptoms.en
dc.description.statusPeer revieweden
dc.description.versionPublished Versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.articleid9879
dc.identifier.citationMancabelli, L., Milani, C., Lugli, G. A., Turroni, F., Mangifesta, M., Viappiani, A., Ticinesi, A., Nouvenne, A., Meschi, T., van Sinderen, D. and Ventura, M. (2017) 'Unveiling the gut microbiota composition and functionality associated with constipation through metagenomic analyses', Scientific Reports, 7(1), 9879 (9pp). doi: 10.1038/s41598-017-10663-wen
dc.identifier.doi10.1038/s41598-017-10663-w
dc.identifier.issn2045-2322
dc.identifier.journaltitleScientific Reportsen
dc.identifier.urihttps://hdl.handle.net/10468/4813
dc.identifier.volume7
dc.language.isoenen
dc.publisherNature Publishing Groupen
dc.relation.projectinfo:eu-repo/grantAgreement/SFI/SFI Research Centres/12/RC/2273/IE/Alimentary Pharmabiotic Centre (APC) - Interfacing Food & Medicine/
dc.relation.projectinfo:eu-repo/grantAgreement/EC/H2020::CSA/696300/EU/The second coordination and support action for the JPI Healthy Diet for a Healthy Life/CSA JPI HDHL 2.0
dc.relation.urihttps://www.nature.com/articles/s41598-017-10663-w
dc.rights© 2017, the Authors. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.en
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjectConstipationen
dc.subjectMetagenomicsen
dc.titleUnveiling the gut microbiota composition and functionality associated with constipation through metagenomic analysesen
dc.typeArticle (peer-reviewed)en
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