Microbial population changes in decaying Ascophyllum nodosum result in Macroalgal-Polysaccharide-Degrading bacteria with potential applicability in enzyme-assisted extraction technologies

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dc.contributor.authorIhua, Maureen W.
dc.contributor.authorGuihéneuf, Freddy
dc.contributor.authorMohammed, Halimah
dc.contributor.authorMargassery, Lekha M.
dc.contributor.authorJackson, Stephen A.
dc.contributor.authorStengel, Dagmar B.
dc.contributor.authorClarke, David J.
dc.contributor.authorDobson, Alan D. W.
dc.contributor.funderScience Foundation Irelanden
dc.contributor.funderDepartment of Agriculture, Food and the Marineen
dc.contributor.funderEuropean Commissionen
dc.contributor.funderMarine Biotechnology ERA/NETen
dc.contributor.funderBeaufort Marine Researchen
dc.contributor.funderMarine Instituteen
dc.contributor.funderNational Development Plan 2007-2013en
dc.date.accessioned2019-11-19T10:18:18Z
dc.date.available2019-11-19T10:18:18Z
dc.date.issued2019-03-29
dc.description.abstractSeaweeds are of significant interest in the food, pharmaceutical, and agricultural industries as they contain several commercially relevant bioactive compounds. Current extraction methods for macroalgal-derived metabolites are, however, problematic due to the complexity of the algal cell wall which hinders extraction efficiencies. The use of advanced extraction methods, such as enzyme-assisted extraction (EAE), which involve the application of commercial algal cell wall degrading enzymes to hydrolyze the cell wall carbohydrate network, are becoming more popular. Ascophyllum nodosum samples were collected from the Irish coast and incubated in artificial seawater for six weeks at three different temperatures (18 °C, 25 °C, and 30 °C) to induce decay. Microbial communities associated with the intact and decaying macroalga were examined using Illumina sequencing and culture-dependent approaches, including the novel ichip device. The bacterial populations associated with the seaweed were observed to change markedly upon decay. Over 800 bacterial isolates cultured from the macroalga were screened for the production of algal cell wall polysaccharidases and a range of species which displayed multiple hydrolytic enzyme activities were identified. Extracts from these enzyme-active bacterial isolates were then used in EAE of phenolics from Fucus vesiculosus and were shown to be more efficient than commercial enzyme preparations in their extraction efficiencies.en
dc.description.sponsorshipNEPTUNA project; SMI-BIO project; FIRM 1/F009/MabSen
dc.description.statusPeer revieweden
dc.description.versionPublished Versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.articleid200en
dc.identifier.citationIhua, M.W., Guihéneuf, F., Mohammed, H., Margassery, L.M., Jackson, S.A., Stengel, D.B., Clarke, D.J. and Dobson, A.D., 2019. Microbial Population Changes in Decaying Ascophyllum nodosum Result in Macroalgal-Polysaccharide-Degrading Bacteria with Potential Applicability in Enzyme-Assisted Extraction Technologies. Marine Drugs, 17(4), (200). DOI:10.3390/md17040200en
dc.identifier.doi10.3390/md17040200en
dc.identifier.eissn1660-3397
dc.identifier.endpage20en
dc.identifier.issn1660-3397
dc.identifier.issued4en
dc.identifier.journaltitleMarine Drugsen
dc.identifier.startpage1en
dc.identifier.urihttps://hdl.handle.net/10468/9058
dc.identifier.volume17en
dc.language.isoenen
dc.publisherMDPIen
dc.relation.projectinfo:eu-repo/grantAgreement/EC/FP7::SP1::KBBE/312184/EU/Increasing Value and Flow in the Marine Biodiscovery Pipeline/PHARMASEAen
dc.relation.projectinfo:eu-repo/grantAgreement/SFI/SFI Research Centres/12/RC/2275/IE/Synthesis and Solid State Pharmaceutical Centre (SSPC)/en
dc.relation.urihttps://www.mdpi.com/1660-3397/17/4/200
dc.rights© 2019 by the authors. Licensee MDPI, Basel, Switzerlanden
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/en
dc.subjectAscophyllum nodosumen
dc.subjectAlgal cell wall degrading enzymesen
dc.subjectEnzyme-assisted extractionen
dc.subjectIchip deviceen
dc.titleMicrobial population changes in decaying Ascophyllum nodosum result in Macroalgal-Polysaccharide-Degrading bacteria with potential applicability in enzyme-assisted extraction technologiesen
dc.typeArticle (peer-reviewed)en
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