Functional and practical insights into three lactococcal antiphage systems

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dc.check.date2025-02-13en
dc.check.infoAccess to this article is restricted until 6 months after publication by request of the publisheren
dc.contributor.authorGrafakou, Andrianaen
dc.contributor.authorMosterd, Casen
dc.contributor.authorde Waal, Paul P.en
dc.contributor.authorvan Rijswijck, Irma M. H.en
dc.contributor.authorvan Peij, Noël N. M. E.en
dc.contributor.authorMahony, Jenniferen
dc.contributor.authorvan Sinderen, Douween
dc.contributor.editorErcolini, Daniloen
dc.contributor.funderScience Foundation Irelanden
dc.date.accessioned2024-10-10T15:00:02Z
dc.date.available2024-10-10T15:00:02Z
dc.date.issued2024-08-13en
dc.description.abstractThe persistent challenge of phages in dairy fermentations requires the development of starter cultures with enhanced phage resistance. Recently, three plasmid-encoded lactococcal antiphage systems, named Rhea, Aristaios, and Kamadhenu, were discovered. These systems were found to confer high levels of resistance against various Skunavirus members. In the present study, their effectiveness against phage infection was confirmed in milk-based medium, thus validating their potential to ensure reliable dairy fermentations. We furthermore demonstrated that Rhea and Kamadhenu do not directly hinder phage genome replication, transcription, or associated translation. Conversely, Aristaios was found to interfere with phage transcription. Two of the antiphage systems are encoded on pMRC01-like conjugative plasmids, and the Kamadhenu-encoding plasmid was successfully transferred by conjugation to three lactococcal strains, each of which acquired substantially enhanced phage resistance against Skunavirus members. Such advances in our knowledge of the lactococcal phage resistome and the possibility of mobilizing these protective functions to bolster phage protection in sensitive strains provide practical solutions to the ongoing phage problem in industrial food fermentations.en
dc.description.sponsorshipScience Foundation Ireland (12/RC/2273‐P1; 12/RC/2273‐P2)en
dc.description.statusPeer revieweden
dc.description.versionPublished Versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.articleide01120-24en
dc.identifier.citationGrafakou, A., Mosterd, C., de Waal, P. P., van Rijswijck, I. M., van Peij, N. N., Mahony, J. and van Sinderen, D. (2024) 'Functional and practical insights into three lactococcal antiphage systems', Applied and Environmental Microbiology, 90(9), e01120-24 (15pp). https://doi.org/10.1128/aem.01120-24en
dc.identifier.doihttps://doi.org/10.1128/aem.01120-24en
dc.identifier.eissn1098-5336en
dc.identifier.endpage15en
dc.identifier.issn0099-2240en
dc.identifier.issued9en
dc.identifier.journaltitleApplied and Environmental Microbiologyen
dc.identifier.startpage1en
dc.identifier.urihttps://hdl.handle.net/10468/16513
dc.identifier.volume90en
dc.language.isoenen
dc.publisherAmerican Society for Microbiologyen
dc.relation.projectinfo:eu-repo/grantAgreement/SFI/SFI Spokes Programme::Rolling Call/17/SP/4678/IE/BacTrans – Natural DNA Transfer Systems for Bacterial Starter Cultures/en
dc.rights© 2024, American Society for Microbiology. All Rights Reserved.en
dc.subjectLactococcusen
dc.subjectLactic acid bacteriaen
dc.subjectBacterial immunityen
dc.subjectPhage defenseen
dc.subjectAbien
dc.titleFunctional and practical insights into three lactococcal antiphage systemsen
dc.typeArticle (peer-reviewed)en
oaire.citation.issue9en
oaire.citation.volume90en
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