dc.contributor.author	Culligan, Eamonn P.
dc.contributor.author	Sleator, Roy D.
dc.contributor.author	Marchesi, Julian R.
dc.contributor.author	Hill, Colin
dc.contributor.funder	Science Foundation Ireland
dc.contributor.funder	APC Microbiome Institute, College of Medicine and Health, University College Cork
dc.contributor.funder	Royal Society, United Kingdom
dc.contributor.funder	European Society of Clinical Microbiology and Infectious Diseases, Switzerland
dc.date.accessioned	2016-02-17T11:44:41Z
dc.date.available	2016-02-17T11:44:41Z
dc.date.issued	2013
dc.description.abstract	Functional environmental screening of metagenomic libraries is a powerful means to identify and assign function to novel genes and their encoded proteins without any prior sequence knowledge. In the current study we describe the identification and subsequent analysis of a salt-tolerant clone from a human gut metagenomic library. Following transposon mutagenesis we identified an unknown gene (stlA, for "salt tolerance locus A") with no current known homologues in the databases. Subsequent cloning and expression in Escherichia coli MKH13 revealed that stlA confers a salt tolerance phenotype in its surrogate host. Furthermore, a detailed in silico analysis was also conducted to gain additional information on the properties of the encoded StlA protein. The stlA gene is rare when searched against human metagenome datasets such as MetaHit and the Human Microbiome Project and represents a novel and unique salt tolerance determinant which appears to be found exclusively in the human gut environment.	en
dc.description.sponsorship	Science Foundation Ireland (CSET Uplift Grant); European Society of Clinical Microbiology and Infectious Diseases, Switzerland (Research Fellowship)	en
dc.description.status	Peer reviewed	en
dc.description.version	Published Version	en
dc.format.mimetype	application/pdf	en
dc.identifier.articleid	e82985
dc.identifier.citation	Culligan EP, Sleator RD, Marchesi JR, Hill C (2013) Functional Environmental Screening of a Metagenomic Library Identifies stlA; A Unique Salt Tolerance Locus from the Human Gut Microbiome . PLoS ONE 8(12): e82985. doi:10.1371/journal.pone.0082985
dc.identifier.doi	10.1371/journal.pone.0082985
dc.identifier.issn	1932-6203
dc.identifier.issued	12	en
dc.identifier.journaltitle	PLOS ONE	en
dc.identifier.uri	https://hdl.handle.net/10468/2350
dc.identifier.volume	8	en
dc.language.iso	en	en
dc.publisher	Public Library of Science	en
dc.rights	© 2015 Culligan et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited	en
dc.rights.uri	http://creativecommons.org/licenses/by/4.0/	en
dc.subject	Outer membrane protein	en
dc.subject	Structure prediction	en
dc.subject	Osmotic stress	en
dc.subject	Benzodiazepine receptor	en
dc.subject	Akkermansia muciniphila	en
dc.subject	Gastrointestinal tract	en
dc.subject	Sequence analysis	en
dc.subject	Escherichia coli	en
dc.subject	Ankyrin repeat	en
dc.subject	I-TASSER	en
dc.title	Functional environmental screening of a metagenomic library identifies stlA; a unique salt tolerance locus from the human gut microbiome	en
dc.type	Article (peer-reviewed)	en

Functional environmental screening of a metagenomic library identifies stlA; a unique salt tolerance locus from the human gut microbiome

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