Functional environmental screening of a metagenomic library identifies stlA; a unique salt tolerance locus from the human gut microbiome
dc.contributor.author | Culligan, Eamonn P. | |
dc.contributor.author | Sleator, Roy D. | |
dc.contributor.author | Marchesi, Julian R. | |
dc.contributor.author | Hill, Colin | |
dc.contributor.funder | Science Foundation Ireland | |
dc.contributor.funder | APC Microbiome Institute, College of Medicine and Health, University College Cork | |
dc.contributor.funder | Royal Society, United Kingdom | |
dc.contributor.funder | European Society of Clinical Microbiology and Infectious Diseases, Switzerland | |
dc.date.accessioned | 2016-02-17T11:44:41Z | |
dc.date.available | 2016-02-17T11:44:41Z | |
dc.date.issued | 2013 | |
dc.description.abstract | Functional environmental screening of metagenomic libraries is a powerful means to identify and assign function to novel genes and their encoded proteins without any prior sequence knowledge. In the current study we describe the identification and subsequent analysis of a salt-tolerant clone from a human gut metagenomic library. Following transposon mutagenesis we identified an unknown gene (stlA, for "salt tolerance locus A") with no current known homologues in the databases. Subsequent cloning and expression in Escherichia coli MKH13 revealed that stlA confers a salt tolerance phenotype in its surrogate host. Furthermore, a detailed in silico analysis was also conducted to gain additional information on the properties of the encoded StlA protein. The stlA gene is rare when searched against human metagenome datasets such as MetaHit and the Human Microbiome Project and represents a novel and unique salt tolerance determinant which appears to be found exclusively in the human gut environment. | en |
dc.description.sponsorship | Science Foundation Ireland (CSET Uplift Grant); European Society of Clinical Microbiology and Infectious Diseases, Switzerland (Research Fellowship) | en |
dc.description.status | Peer reviewed | en |
dc.description.version | Published Version | en |
dc.format.mimetype | application/pdf | en |
dc.identifier.articleid | e82985 | |
dc.identifier.citation | Culligan EP, Sleator RD, Marchesi JR, Hill C (2013) Functional Environmental Screening of a Metagenomic Library Identifies stlA; A Unique Salt Tolerance Locus from the Human Gut Microbiome . PLoS ONE 8(12): e82985. doi:10.1371/journal.pone.0082985 | |
dc.identifier.doi | 10.1371/journal.pone.0082985 | |
dc.identifier.issn | 1932-6203 | |
dc.identifier.issued | 12 | en |
dc.identifier.journaltitle | PLOS ONE | en |
dc.identifier.uri | https://hdl.handle.net/10468/2350 | |
dc.identifier.volume | 8 | en |
dc.language.iso | en | en |
dc.publisher | Public Library of Science | en |
dc.rights | © 2015 Culligan et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited | en |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | en |
dc.subject | Outer membrane protein | en |
dc.subject | Structure prediction | en |
dc.subject | Osmotic stress | en |
dc.subject | Benzodiazepine receptor | en |
dc.subject | Akkermansia muciniphila | en |
dc.subject | Gastrointestinal tract | en |
dc.subject | Sequence analysis | en |
dc.subject | Escherichia coli | en |
dc.subject | Ankyrin repeat | en |
dc.subject | I-TASSER | en |
dc.title | Functional environmental screening of a metagenomic library identifies stlA; a unique salt tolerance locus from the human gut microbiome | en |
dc.type | Article (peer-reviewed) | en |
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